Neurofilament 150, 200kD antibody
Reactivity: Human, Mouse, Rabbit, Rat
IHC, WB, IHC (p), IP, ELISA, IHC (fro)
Host: Mouse
Monoclonal
unconjugated
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- Target
- Neurofilament 150, 200kD
- Reactivity
- Human, Mouse, Rabbit, Rat
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- Un-conjugated
- Application
- Immunohistochemistry (IHC), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro))
- Specificity
- Recognizes the 160 alpha 200kD proteins of human neurofilament. Specifically recognizes a non-phosphorylated epitope in the tail domain of NF-M+H. Dephosphorylation of NF-M+H will increase immunoreactivity with this antibody. Species cross-reactivity: human, rat, mouse, bovine, rabbit, hamster and squid.
- Purification
- Purified
- Immunogen
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Adult rat neurofilament.
Type of Immunogen: Protein - Isotype
- IgG1 kappa
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anti-Neurofilament 150, 200kD antibody
Reactivity: Human IHC, WB Host: Mouse Monoclonal 13AA unconjugated
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- Application Notes
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Approved: ELISA (0.1 - 0.5 μg/mL), IHC, IHC-Fr, IHC-P, IP, WB (0.5 - 1 μg/mL)
Usage: Suitable for use in ELISA, Immunohistochemistry, Immunoprecipitation, and Western Blot. ELISA: 0.1-0.5 μg/mL. Immunohistochemistry (Bouin's and alcohol-fixed, paraffin-embedded sections and frozen sections): 5-10 μg/mL. To stain, incubate 30-60 minutes at RT or overnight at 4?C. Western Blot: 0.5-1 μg/mL. Immunoprecipitation: 2-5 μg. - Comment
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Target Species of Antibody: Rat
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, 0.05 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- Target
- Neurofilament 150, 200kD
- Alternative Name
- Neurofilament 150, 200kD (NEFM/NEFH)
- Background
- This antibody detects: NEFM + NEFH
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