Neurofilament 150, 200kD antibody

Catalog No. ABIN479504

The Mouse Monoclonal anti-Neurofilament 150, 200kD antibody is suitable to detect Neurofilament 150, 200kD in samples from Human, Mouse, Rabbit and Rat. It has been validated for IHC, WB, ELISA, IHC (fro), IHC (p) and IP.

Quick Overview for Neurofilament 150, 200kD antibody (ABIN479504)

Target: Neurofilament 150, 200kD

Reactivity: Human, Mouse, Rabbit, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Product Details

Specificity: Recognizes the 160 alpha 200kD proteins of human neurofilament. Specifically recognizes a non-phosphorylated epitope in the tail domain of NF-M+H. Dephosphorylation of NF-M+H will increase immunoreactivity with this antibody. Species cross-reactivity: human, rat, mouse, bovine, rabbit, hamster and squid.

Purification: Purified

Immunogen: Adult rat neurofilament.

Type of Immunogen: Protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA (0.1 - 0.5 μg/mL), IHC, IHC-Fr, IHC-P, IP, WB (0.5 - 1 μg/mL)

Usage: Suitable for use in ELISA, Immunohistochemistry, Immunoprecipitation, and Western Blot. ELISA: 0.1-0.5 μg/mL. Immunohistochemistry (Bouin's and alcohol-fixed, paraffin-embedded sections and frozen sections): 5-10 μg/mL. To stain, incubate 30-60 minutes at RT or overnight at 4?C. Western Blot: 0.5-1 μg/mL. Immunoprecipitation: 2-5 μg.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details

Target: Neurofilament 150, 200kD

Alternative Name: Neurofilament 150, 200kD (NEFM/NEFH)

Background: This antibody detects: NEFM + NEFH