SSEA-4 antibody

Catalog No. ABIN480275

The Mouse Monoclonal anti-SSEA-4 antibody has been validated for FACS, IHC and IHC (fro). It is suitable to detect SSEA-4 in samples from Human and Mouse.

Quick Overview for SSEA-4 antibody (ABIN480275)

Target: SSEA-4 (Thiosulfate Sulfurtransferase 4 (SSEA-4))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This SSEA-4 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-SSEA-4 Antibody

Specificity: Recognizes a carbohydrate epitope of human SSEA-4. Species cross-reactivity: Mouse.

Purification: Protein A purified

Immunogen: Human embryonal carcinoma cell line 2102 Ep.

Type of Immunogen: Cells

Isotype: IgG3

Application Details

Application Notes: Approved: Flo, IHC, IHC-Fr

Usage: Flow Cytometry, Immunohistochemistry. Immunohistochemistry: This antibody has been used with the appropriate secondary reagents at a concentration of 5-10 μg/mL in fixed cells. Cells were fixed with 4 % paraformaldehyde in PBS at room temperature for 20 minutes, then blocked with PBS containing 10 % normal donkey serum and 1 % BSA at room temperature for 45 minutes. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by Rhodamine Red coupled anti-mouse IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. This antibody can also be used in unfixed, shock frozen tissue at a concentration of 10 μg/mL. Flow Cytometry: Dilute this antibody to 0.1 mg/mL and add 5 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 μL of a 25 μg/mL stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution: Reconstitute, sterile PBS. If 0.2 mL of PBS is used, the antibody concentration will be 500 μg/mL.

Concentration: Lot specific

Buffer: Lyophilized from TBS, 5 % trehalose. Reconstitution: Reconstitute, sterile PBS. If 0.2 mL of PBS is used, the antibody concentration will be 500 μg/mL.

Target Details for SSEA-4

Target: SSEA-4 (Thiosulfate Sulfurtransferase 4 (SSEA-4))

Alternative Name: SSEA-4