IFNGR1 antibody (Extracellular Domain)

Catalog No. ABIN480396

This anti-IFNGR1 antibody is a Goat Polyclonal antibody detecting IFNGR1 in WB, IHC, ELISA, ICC, Neut and Func. Suitable for Human.

Quick Overview for IFNGR1 antibody (Extracellular Domain) (ABIN480396)

Target: IFNGR1 (Interferon gamma Receptor 1 (IFNGR1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This IFNGR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Neutralization (Neut), Functional Studies (Func)

Product Details anti-IFNGR1 Antibody

Binding Specificity: Extracellular Domain

Specificity: Binds to and neutralizes human Interferon gamma receptor 1, 15.756kD. Blocks cell surface human Interferon gamma receptor 1-mediated bioactivity of recombinant human Interferon gamma receptor 1. In direct ELISA and Western Blot, does not cross-react with human Interferon gamma receptor chain2.

Purification: Immunoaffinity purified

Immunogen: Recombinant, human Interferon gamma receptor chain 1, NS0-derived, extracellular domain.

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, Func, ICC, IHC (15 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)

Usage: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Neutralization. Neutralization of Human IFN-g R1 Bioactivity: The exact concentration of antibody required to neutralize rhIFN-g R1-mediated bioactivity is dependent on the cytokine concentration, cell type, growth conditions and the type of activity studied. The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when that cytokine is present at a concentration just high enough to elicit a maximum response. The ND50 is ~0.5-2 μg/mL in the presence of 2 ng/mL of rhIFN-g, using a confluent culture of HeLa cells. ELISA (Direct): 0.5-1 μg/mL. The detection limit for rhIFN-g R1 is ~0.3 ng/well. Western Blot: 0.1-0.2 μg/mL. The detection limit for is ~5 ng/lane under non-reducing and reducing conditions. Immunohistochemistry: 15 μg/mL. Detects IFN-g R1 in human PBMC's (peripheral blood mononuclear cells) (fluorescence immunocytochemistry).

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 5 % trehalose. No preservative added.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for IFNGR1

Target: IFNGR1 (Interferon gamma Receptor 1 (IFNGR1))

Alternative Name: CD119 / IFNGR1

Background: Name/Gene ID: IFNGR1
Family: Interferon Receptor

Synonyms: IFNGR1, AVP, type 2, Antiviral protein, type 2, CD119, CD119 antigen, IFNGR, IFN-gamma receptor 1, IFN-gamma-R1, Immune interferon receptor 1, Interferon gamma receptor 1, CDw119

Gene ID: 3459

UniProt: P15260

Pathways: Interferon-gamma Pathway