CXCL5 antibody

Catalog No. ABIN480885

This anti-CXCL5 antibody is a Goat Polyclonal antibody detecting CXCL5 in WB, ELISA, IHC, IHC (p), Neut and Func. Suitable for Human.

Quick Overview for CXCL5 antibody (ABIN480885)

Target: CXCL5 (Chemokine (C-X-C Motif) Ligand 5 (CXCL5))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CXCL5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Neutralization (Neut), Functional Studies (Func)

Product Details anti-CXCL5 Antibody

Specificity: Recognizes human CXCL5. Neutralizes human CXCL5 bioactivity. Based on direct ELISA results, this antibody shows less than 20 % cross-reactivity with recombinant, human GCP-2 and less than 5 % cross-reactivity with recombinant, mouse KC, recombinant, rat CINC-1, recombinant, human GRO, recombinant, human GRO and recombinant, mouse GCP-2. Additionally, in direct ELISA, this antibody shows no cross-reactivity with other chemokines tested.

Purification: Immunoaffinity purified

Immunogen: E. coli-derived Recombinant, Human CXCL5.

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), Func, IHC, IHC-P (0.1 - 0.2 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)

Usage: Suitable for use in ELISA, Western Blot, Neutralization, Immunohistochemistry. Western Blot: 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human CXCL5. The detection limit for Recombinant, Human CXCL5 is approximately 5 ng/lane under non-reducing and reducing conditions. Neutralization of Human CXCL5 Bioactivity. The exact concentration of antibody required to neutralize rhCXCL5 activity is dependent on the cytokine concentration, cell type, growth conditions and the type of activity studied. The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when that cytokine is present at a concentration just high enough to elicit a maximum response. The ND50 for this lot of anti-human CXCL5 antibody was determined to be approximately 0.2-1 μg/mL in the presence of 30 ng/mL of rhCXCL5, using BaF/3 cells transfected with rhCXCR-2. Direct ELISA: 0.5-1 μg/mL with the appropriate secondary reagents to detect human CXCL5. The detection limit for Recombinant, Human CXCL5 is approximately 0.15 ng/well. Immunohistochemistry: 15 μg/mL Paraffin-embedded tissue sections. To measure the ability of the antibody to neutralize the chemoattractant activity of rhCXCL5 for hCXCR-2 transfected BaF/3cells, Recombinant, Human CXCL5 was incubated with various concentrations of the antibody for 15 minutes at RT in a 96 well microplate. Following this preincubation period, 75 μL of the cytokine-antibody solution (containing rhCXCL5 at a final concentration of 0.03g/mL and antibody at the concentrations indicated) was transferred to the lower compartment of a 96 well chemotaxis chamber (NeuroProbe, Cabin John, MD). The chemotaxis chamber was then assembled using a PVP-free polycarbonate filter (5 micron pore size) and 0.2 x1 x10⁶/well was added to the top chamber. After incubation for 3 hours at 37? C in a 5 % CO2. humidified incubator, the chamber was carefully disassembled. The cells that migrate through to the lower chamber were transferred to a 96 well plate. Chemotaxis was measured by Resazurin staining of cells that have migrated through the filter. As shown in figure 2, the ND50 for this lot of antibody is approximately 0.2-1 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Reconstitute with sterile PBS

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 5 % trehalose.

Storage: -20 °C

Storage Comment: Lyophilized and resuspended forms: store at -20°C short term.

Target Details for CXCL5

Target: CXCL5 (Chemokine (C-X-C Motif) Ligand 5 (CXCL5))

Alternative Name: CXCL5

Background: Name/Gene ID: CXCL5
Subfamily: Intercrine alpha
Family: Intercrine

Synonyms: CXCL5, C-X-C motif chemokine 5, ENA-78(1-78), GCP-2, ENA78, SCYB5, ENA-78, Small inducible cytokine B5, Small-inducible cytokine B5, LIX, AMCF-II

Gene ID: 6374

UniProt: P42830

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity