CCL4 antibody

Catalog No. ABIN480888

This anti-CCL4 antibody is a Goat Polyclonal antibody detecting CCL4 in WB, ELISA, IHC and Neut. Suitable for Human and Mouse.

Quick Overview for CCL4 antibody (ABIN480888)

Target: CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This CCL4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Neutralization (Neut)

Product Details anti-CCL4 Antibody

Specificity: Recognizes Macrophage Inflammatory Protein 1 beta (MIP1 beta). based on ELISA and Western Blot data, <10 % cross-reactivity with mouse MIP-1b, human MIP-1b, human MCP-2 and <5 % cross-reactivity with mouse MIP-1b. <1 % cross-reactivity with other chemokines tested.

Purification: Immunoaffinity purified

Immunogen: Sf 21-derived Recombinant, human Macrophage Inflammatory Protein 1 beta (MIP1 beta, CCL4).

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), IHC (0.5 - 5 μg/mL), Neut (0.3 - 1.5 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Suitable for use in neutralization of bioactivity, ELISA, Western Blot, and Immunohistochemistry. Neutralization: 0.3-1.5 μg/mL in the presence of 0.04 μg/mL of rhMIP-1b, using the BaF/3 hCCR5 transfected cell chemotaxis assay. In chemotaxis assays, this antibody will neutralize the biological activity of human MIP-1b and mouse MIP-1b at a 25-fold higher IgG concentration. Will not neutralize the biological activity of mouse MIP1b. ELISA: 0.5-1 μg/mL with the appropriate secondary reagents to detect human MIP-1b. The detection limit is ~0.3 ng/well. Western Blot: 0.1-0.2 μg/mL. The detection limit is 1.5-3.0 ng/lane, under non-reducing and reducing conditions. Immunohistochemistry: 0.5-5 μg/mL in cultured cells or tissue. sections.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 40 % glycerol.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for CCL4

Target: CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))

Alternative Name: CCL4 / SYCA4

Background: Name/Gene ID: CCL4
Subfamily: Intercrine beta
Family: Intercrine

Synonyms: CCL4, ACT2, Act-2, AT744.1, C-C motif chemokine 4, Chemokine (C-C motif) ligand 4, CC chemokine ligand 4, HC21, LAG1, MIP1B, MIP1B1, LAG-1, SCYA2, Secreted protein G-26, SIS-gamma, MIP-1-beta, MIP-1-beta(1-69), Small-inducible cytokine A4, T-cell activation protein 2, G-26, Lymphocyte-activation gene 1, PAT 744, Protein H400, SCYA4

Gene ID: 6351

UniProt: P13236