Macrophages antibody

Catalog No. ABIN481657

This Mouse Monoclonal antibody specifically detects Macrophages in IHC (fro) and IHC. It exhibits reactivity toward Human.

Quick Overview for Macrophages antibody (ABIN481657)

Target: Macrophages

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (IHC)

Clone: PM-2K

Product Details

Specificity: The antigen of 150  kDa m.w. is found in the macrophage cell membrane. The epitope has not been determined. Antigen distribution: Isolated cells: Positive on >90 % of alveolar macrophages, and on 10 % of adherent peritoneal cells after 1 day culture. Negative on dendritic cells, on freshly isolated blood monocytes or peritoneal cells, and on bone marrow cells including monocytes, myelomonocytic precursors and megakaryocytes. Tissue sections: PM-2K stains most tissue macrophages in lymphoreticular organs such as thymus, spleen, lymph node and tonsil. It is positive on Kupffer cells of the liver, alveolar macrophages and macrophages in the interstitial tissues of the kidney, pancreas and many other organs. In Gaucher's disease multicentric reticulohistiocytosis and malignant histiocytosis proliferating macrophages are positive. In MFH infiltrating macrophages are stained but not tumour cells. Some bone stromal cells but not osteoclast-like multinucleated giant cells are stained in GCT. Microglial cells, osteoclasts and dendritic cells such as Langerhans cells, interdigitating cells and follicular dendritic cells are negative. Specificity: Human: Macrophages. Other: positive on macrophages of cat, dog, pig, bovine and monkey, negative in rabbit, rat, guinea pig, gold hamster, goat, horse.

Purification: Affinity purified

Immunogen: Cultured human peritoneal macrophages

Type of Immunogen: Cells

Application Details

Application Notes: Approved: IHC, IHC-Fr (1:400)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.5 mL distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS  pH 7.2, 10 mg/mL BSA, 0.01 % thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 4°C.

Target Details

Target: Macrophages