Macrophages antibody

Catalog No. ABIN481694

The Mouse Monoclonal anti-Macrophages antibody is suitable to detect Macrophages in samples from Rat. It has been validated for IHC (p) and IHC.

Quick Overview for Macrophages antibody (ABIN481694)

Target: Macrophages

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)

Clone: Ki-M2R

Product Details

Specificity: Ki-M2R recognizes a surface antigen of 45kD molecular weight as a single band under reducing conditions. The epitope has not been further characterized Antigen distribution: Isolated cells: In the bone marrow a minor number of macrophages reveal a strong reactivity whereas granulocytes, erythropoetic cells and megacaryocytes are negative. Immature accessory cells such as dendritic or interdigitating reticulum cells are also negative. Monocytes show a positive reaction when exposed to TPA or foreign material. Tissue sections: Ki-M2R positive cells have been recognised as active phagocytes in serous cavities (peritoneal and pleural macrophages). Liver (Kupffer cells), spleen (macrophages of the red pulp), lymph nodes (macrophages of the pulp and sinus, tingible body macrophages), lung (approx. 50 % of alveolar macrophages) and connective tissue (histiocytes) are positively stained. Plasma cells (excluding stimulated monocytes) as well as sinus lining cells are negative. Specificity: Rat: mature macrophages. Other species: not tested

Purification: Affinity purified

Immunogen: Peritoneal macrophages

Application Details

Application Notes: Approved: IHC, IHC-P (1:400)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.5 mL distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS  pH 7.2, 5 mg/mL BSA, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 4°C.

Target Details

Target: Macrophages