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AMY2A antibody (N-Term)

The Mouse Monoclonal anti-AMY2A antibody has been validated for WB and EIA. It is suitable to detect AMY2A in samples from Rat.
Catalog No. ABIN487412

Quick Overview for AMY2A antibody (N-Term) (ABIN487412)

Target

See all AMY2A Antibodies
AMY2A (Amylase, alpha 2A (Pancreatic) (AMY2A))

Reactivity

  • 50
  • 24
  • 4
  • 1
  • 1
Rat

Host

  • 51
  • 5
  • 1
Mouse

Clonality

  • 53
  • 4
Monoclonal

Conjugate

  • 31
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This AMY2A antibody is un-conjugated

Application

  • 48
  • 17
  • 16
  • 13
  • 13
  • 11
  • 10
  • 7
  • 4
  • 3
  • 2
Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone

RPA-B5
  • Binding Specificity

    • 15
    • 9
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1-492, N-Term

    Specificity

    Specifically recognizes Rat Pancreatic Amylase.

    Cross-Reactivity (Details)

    Species reactivity (tested):Rat.

    Characteristics

    Synonyms: AMY2A, Pancreatic alpha-amylase, 1, 4-alpha-D-glucan glucanohydrolase 2A

    Purification

    Affinity Chromatography.

    Immunogen

    Recombinant protein (1-492 amino acids of the CDS) expressed in E. coli.

    Isotype

    IgG1
  • Application Notes

    Indirect ELISA (1-2 μg/mL): Detect up to 20 ng of coated antigen. Western blotting (approx. 4 μg/mL): Detects Rat Pancreatic Amylase at the expected MolecularWeight at approx. 54 kDa.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Concentration

    0.5 mg/mL

    Buffer

    Phosphate-buffered saline (PBS) containing 30 % Glycerol, 0.5 % BSA and and 0.01 % Thimerosal.

    Preservative

    Thimerosal (Merthiolate)

    Precaution of Use

    This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    -20 °C

    Storage Comment

    Store the antibody (in aliquots) at -20 °CAvoid repeated freezing and thawing.
    Shelf life: one year from despatch.

    Expiry Date

    12 months
  • Target

    AMY2A (Amylase, alpha 2A (Pancreatic) (AMY2A))

    Background

    Pancreatic Amylase is a digestive enzyme secreted by pancreas into the intestine. It consists of a single polypeptide chain with a molecular weight of 54 kDa. Measurement of Amylase concentrations in serum is a diagnostic test for various pancreatic disorders.Synonyms: 1, 4-alpha-D-glucan glucanohydrolase 2A, AMY2A, Pancreatic alpha-amylase

    Gene ID

    497039

    UniProt

    P00689
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