ITGA7 antibody

Catalog No. ABIN487465

This Mouse Monoclonal antibody specifically detects ITGA7 in FACS and Neut. It exhibits reactivity toward Mouse.

Quick Overview for ITGA7 antibody (ABIN487465)

Target: ITGA7 (Integrin, alpha 7 (ITGA7))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This ITGA7 antibody is un-conjugated

Application: Flow Cytometry (FACS), Neutralization (Neut)

Clone: 6A11

Product Details anti-ITGA7 Antibody

Cross-Reactivity (Details): Species reactivity (tested):Mouse.

Purification: Protein-A Sepharose Chromatography.

Immunogen: Mouse myoblasts. Remarks: Hybridoma was established by fusion of mouse myeloma cell SP2/0 with Integrinalpha-7 knockout C57/B6 Mouse splenocyte

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: 10-20 μg/mL (final concentration). Blocking: more than 10 μg/mLPositive Control: C2C12.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Flow Cytometric Analysis for Floating CellsProtocol 1. We usually use Fisher tubes or equivalents as reaction tubes for all step described below. 1) Detach the cells from culture dishes by tripsinization. 2) Stop the trypsinization by adding medium containing 10% fetal calf serum (FCS). 3) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS)and 0. 1% NaN3]. 4) Add 10 µL of normal goat serum to the cell pellet after tapping. Mix well, and incubatefor 5 minutes at room temperature (20~25°C). 5) Add 30 µL of the anti-mouse integrin alpha-7 monoclonal antibody (6A11) (10-20 µg/mL)diluted with the washing buffer. Mix well, and incubate for 30 minutes at room temperature(20~25°C). 6) Add 1 mL of the washing buffer followed by centrifugation at 500xg for 1 minute at roomtemperature (20~25°C). Remove supernatant by careful aspiration. 7) Add 30 µL of secondary antibody (1: 40 FITC conjugated anti-mouse IgG diluted with thewashing buffer. Mix well and incubate for 15 minutes at room temperature (20~25°C). 8) Add 1 mL of the washing buffer followed by centrifugation at 500xg for 1 minute at roomtemperature (20~25°C). Remove supernatant by careful aspiration. 9) Resuspend the cells with 500 µL of the washing buffer and analyze by a flow cytometer.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.2 containing 50 % Glycerol without preservatives.

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store the antibody undiluted at -20 °C.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for ITGA7

Target: ITGA7 (Integrin, alpha 7 (ITGA7))

Alternative Name: Integrin alpha-7 / ITGA7

Background: The integrin family of adhesion molecules participate in important cell-cell and cell-extracellular matrix interactions in a diverse range of biological processes. Integrins are heterodimers consisting of an alpha subunit and a beta subunit. Both alpha and beta subunits are transmembrane proteins with large extracellular domains (>100 kDa for alpha subunit and >75 kDa for beta subunit) that interact with extracellular matrix proteins and relatively small cytoplasmic domains (50 amino acids or less, except for the beta-4 subunit) that interact with cytoskeletal proteins. The adhesiveness of integrins is dynamically regulated in response to cytoplasmic signals, termed

UniProt: Q61738

Pathways: Integrin Complex