Digoxigenin antibody

Catalog No. ABIN487658

This Sheep Polyclonal antibody specifically detects Digoxigenin in EIA. It exhibits reactivity toward Digitalis purpurea (Common foxglove) and Digitalis spec..

Quick Overview for Digoxigenin antibody (ABIN487658)

Target: Digoxigenin (DIG)

Reactivity: Digitalis purpurea (Common foxglove), Digitalis spec.

Host: Sheep

Clonality: Polyclonal

Conjugate: This Digoxigenin antibody is un-conjugated

Application: Enzyme Immunoassay (EIA)

Product Details anti-Digoxigenin Antibody

Purification: Ig Fraction

Immunogen: Digoxigenin-BTG

Isotype: IgG

Application Details

Application Notes: ELISA: 0.625 μg/mL, 10 ng/mL digoxigenin produces 91 % inhibition in a competitive ELISA,employing digoxigenin polyclonal antibody.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 7.00mg/mL (U.V. abs at 280nm)

Buffer: 20 mM Phosphate, 150 mM Sodium Chloride, pH 7.2 containing 0.09 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for Digoxigenin

Target: Digoxigenin (DIG)

Alternative Name: Digoxigenin

Target Type: Chemical

Background: Digoxigenin (DIG) is a steroid found exclusively in the flowers and leaves of the plants Digitalis purpurea and Digitalis lanata. Digoxigenin is chemically closely related to Digoxin, the cardiac glycoside used for the treatment of various heart diseases. The term 'genin' at the end of Digoxigenin, refers to only the aglycone portion (without the sugar) part of the molecule,thus Digoxigenin is the steroid component of Digoxin, - minus the (digitose) sugar residues. DIG can be covalently added to proteins or nucleic acids which makes it very useful in diverse applications.