Methotrexate antibody

Catalog No. ABIN487772

This Sheep Polyclonal antibody specifically detects Methotrexate in EIA. It exhibits reactivity toward .

Quick Overview for Methotrexate antibody (ABIN487772)

Target: Methotrexate (MTX)

Reactivity: Please inquire

Host: Sheep

Clonality: Polyclonal

Conjugate: This Methotrexate antibody is un-conjugated

Application: Enzyme Immunoassay (EIA)

Product Details anti-Methotrexate Antibody

Purification: Ig Fraction

Immunogen: Methotrexate -BSA

Isotype: IgG

Application Details

Application Notes: ELISA: 2.5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 7.74 mg/mL (U.V.abs at 280nm)

Buffer: 20 mM Phosphate, 150 mM Sodium Chloride, pH 7.2 containing 0.09 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for Methotrexate

Target: Methotrexate (MTX)

Alternative Name: Methotrexate

Target Type: Amino Acid

Background: Methotrexate (rINN) abbreviated MTX and formerly known as amethopterin, is an antimetabolite and antifolate drug used in treatment of cancer, autoimmune diseases and as an abortifacient in the induction of medical abortions. It acts by inhibiting the metabolism of folic acid. Methotrexate competitively inhibits dihydrofolate reductase (DHFR), an enzyme that participates in the tetrahydrofolate synthesis. The affinity of methotrexate for DHFR is about one thousand-fold that of folate for DHFR. Methotrexate acts specifically during DNA and RNA synthesis, and thus it is cytotoxic during the S-phase of the cell cycle. Logically, it therefore has a greater toxic effect on rapidly dividing cells which replicate their DNA more frequently, and thus inhibits the growth and proliferation of these non-cancerous cells. Lower doses of methotrexate have been shown to be very effective for the management of rheumatoid arthritis, Crohn's disease, and psoriasis.Synonyms: 4-amino-10-methylpteroyl-L-glutamic acid