Sorting Nexin 1 antibody (Internal Region)

Catalog No. ABIN487988

The Goat Polyclonal anti-Sorting Nexin 1 antibody is suitable to detect Sorting Nexin 1 in samples from Human, Rat, Mouse, Cow and Dog. It has been validated for WB, IF, IHC (p) and EIA.

Quick Overview for Sorting Nexin 1 antibody (Internal Region) (ABIN487988)

Target: Sorting Nexin 1 (SNX1)

Reactivity: Human, Rat, Mouse, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This Sorting Nexin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-Sorting Nexin 1 Antibody

Binding Specificity: Internal Region

Specificity: This antibody is expected to recognize isoform a and c (NP_003090.2, NP_690039.1.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine, Bovine.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Peptide with sequence C-TNSSKPQPTYEELE, from the internal region of the protein sequence according to NP_003090.2, NP_690039.1.

Application Details

Application Notes: Peptide ELISA: 1/64000. Western blot: Approx 75 kDa band observed in Human lysates of cell line HeLa (calculatedMW of 59.5 kDa according to NP_003090.2). The observed molecular weight corresponds toearlier findings with different antibodies from other commercial sources. Recommended concentration: 1 - 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for Sorting Nexin 1

Target: Sorting Nexin 1 (SNX1)

Alternative Name: Sorting Nexin-1 (SNX1)

Background: Sorting nexin 1 is a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This endosomal protein regulates the cell-surface expression of epidermal growth factor receptor. This protein also has a role in sorting protease-activated receptor-1 from early endosomes to lysosomes. This protein may form oligomeric complexes with family members. This gene results in three transcript variants encoding distinct isoforms.

Gene ID: 6642

NCBI Accession: NP_003090

UniProt: Q13596