PRDM1 antibody (C-Term)

Catalog No. ABIN487993

This Goat Polyclonal antibody specifically detects PRDM1 in WB and EIA. It exhibits reactivity toward Human, Mouse, Rat, Dog, Pig and Cow.

Quick Overview for PRDM1 antibody (C-Term) (ABIN487993)

Target: PRDM1 (PR Domain Containing 1, with ZNF Domain (PRDM1))

Reactivity: Human, Mouse, Rat, Dog, Pig, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This PRDM1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-PRDM1 Antibody

Binding Specificity: C-Term

Specificity: This antibody is expected to recognize both reported isoforms (NP_001189.2 and NP_878911.1).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine, Porcine, Bovine.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Peptide with sequence C-KVKQETVEPMDP, from the C Terminus of the protein sequence according to NP_001189.2, NP_878911.1.

Application Details

Application Notes: Peptide ELISA: 1/2000. Western blot: Approx 100 kDa band observed in Human Brain (cerebellum) lysates(calculated MW of 91.0 kDa according to NP_001189.2 and of 76.8 according toNP_878911.1). Recommended concentration: 1 - 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for PRDM1

Target: PRDM1 (PR Domain Containing 1, with ZNF Domain (PRDM1))

Alternative Name: PRDM1 / BLIMP1

Background: PRDM1/Blimp1 is a repressor of beta-interferon gene expression. This action is exerted by binding to the PRDI (positive regulatory domain I element) of the beta-IFN gene promoter. The transcription of this gene is increased upon virus induction.Synonyms: BLIMP-1, Beta-interferon gene positive regulatory domain I-binding factor, PR domain zinc finger protein 1, PR domain-containing protein 1, PRDI-BF1, PRDI-binding factor 1, Positive regulatory domain I-binding factor 1

Gene ID: 639

NCBI Accession: NP_001189

UniProt: O75626

Pathways: Regulation of Muscle Cell Differentiation