CD34 antibody (Biotin)

Catalog No. ABIN488155

This Mouse Monoclonal antibody specifically detects CD34 in FACS. It exhibits reactivity toward Human.

Quick Overview for CD34 antibody (Biotin) (ABIN488155)

Target: CD34

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD34 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Clone: 4H11

Product Details anti-CD34 Antibody (Biotin)

Specificity: This antibody reacts with Class III epitope on CD34 (Mucosialin), a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells, it is gradually lost on progenitor cells. The antibody 4H11[APG] completely blocks binding of Class II antibody QBEnd10 and Class III antibodies BIRMA K3 and 8G12 on KG1a cell line.

Cross-Reactivity (Details): Species reactivity (tested):Human

Characteristics: Synonyms: Hematopoietic progenitor cell marker, Hematopoietic progenitor cell antigen CD34

Immunogen: Permanent human cell line derived from peripheral leucocytes of a patient suffering fromchronic myeloid leukaemia

Isotype: IgG1

Application Details

Application Notes: Indirect immunofluorescence analysis by Flow cytometry (1: 200 as starting point.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeatedfreezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CD34

Target: CD34

Alternative Name: CD34

Background: CD34 is a highly glycosylated monomeric 111-115 kDa surface protein, which is present on many stem cell populations. It is a well established stem cell marker, though its expression on human hematopoietic stem cells is reversible. CD34 probably serves as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions.Synonyms: Hematopoietic progenitor cell antigen CD34, Hematopoietic progenitor cell marker

Gene ID: 947

UniProt: P28906