Bromelain antibody

Catalog No. ABIN488556

The Rabbit Polyclonal anti-Bromelain antibody (ABIN488556) specifically detects Bromelain in ELISA, WB, IF and IHC. The antibody is reactive with Pineapple samples.

Quick Overview for Bromelain antibody (ABIN488556)

Target: Bromelain

Reactivity: Pineapple

Host: Rabbit

Clonality: Polyclonal

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Product Details

Predicted Reactivity: Ananas comosus

Characteristics: Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA, immunoblotting and enzyme inhibition.

Purification: affinity purified

Immunogen: native bromelain isolated and purified from Ananas comosus

Isotype: IgG

Application Details

Application Notes: Recommended Dilution: 1 : 1000-1 : 100 000 (ELISA), (IF), (IHC), (WB)

Comment:

The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution please add 1 mL of sterile destilled water.

Buffer: phosphate buffer saline

Handling Advice: Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details

Target: Bromelain

Background: Bromelain (EC=3.4.22.32) is a cysteine protease isolated from pineapple stem.