Bromelain antibody
Reactivity: Pineapple
ELISA, WB, IF, IHC
Host: Rabbit
Polyclonal
unconjugated
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- Target
- Bromelain
- Reactivity
- Pineapple
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- Un-conjugated
- Application
- ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
- Predicted Reactivity
- Ananas comosus
- Characteristics
- Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA, immunoblotting and enzyme inhibition.
- Purification
- affinity purified
- Immunogen
- native bromelain isolated and purified from Ananas comosus
- Isotype
- IgG
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- Application Notes
- Recommended Dilution: 1 : 1000-1 : 100 000 (ELISA), (IF), (IHC), (WB)
- Comment
-
The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- For reconstitution please add 1 mL of sterile destilled water.
- Buffer
- phosphate buffer saline
- Handling Advice
- Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
- Storage
- -20 °C
- Storage Comment
- Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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- Target
- Bromelain
- Background
- Bromelain (EC=3.4.22.32) is a cysteine protease isolated from pineapple stem.
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