Bromelain antibody (Biotin)
Reactivity: Pineapple
ELISA, WB, IF, IHC
Host: Rabbit
Polyclonal
Biotin
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- Target
- Bromelain
- Reactivity
- Pineapple
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- Biotin
- Application
- ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
- Predicted Reactivity
- Ananas comosus
- Characteristics
- Antibody is labelled with biotin using N-hydroxysuccinimidobiotin. Antibody potency and purity has been evaluated by immunoelectrophoresis, single radialimmunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.
- Purification
- affinity purified
- Immunogen
- native bromelain isolated and purified from Ananas comosus
- Isotype
- IgG
-
-
- Application Notes
- Recommended Dilution: 1 : 5000 - 1 : 50 000 for techniques listed above.
- Comment
-
Biotin/IgG protein molar ration is approximately 6.2. No foreign proteins are added.
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- For reconstitution please add 1 mL of sterile destilled water.
- Buffer
- phosphate buffer saline
- Handling Advice
- Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
- Storage
- -20 °C
-
- Target
- Bromelain
- Background
- Bromelain (EC=3.4.22.32) is a cysteine protease isolated from pineapple stem.
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