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Urease antibody

This anti-Urease antibody is a Rabbit Polyclonal antibody detecting Urease in WB, ELISA, IF and IHC. Suitable for Canavalia ensiformis.
Catalog No. ABIN488562

Quick Overview for Urease antibody (ABIN488562)

Target

Urease (URE)

Reactivity

  • 8
  • 1
  • 1
Canavalia ensiformis

Host

  • 10
Rabbit

Clonality

  • 9
  • 1
Polyclonal

Conjugate

  • 4
  • 4
  • 2
This Urease antibody is un-conjugated

Application

  • 9
  • 8
  • 6
  • 5
  • 4
  • 2
  • 2
  • 2
  • 1
Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC)
  • Characteristics

    Expected / apparent Molecular Weight of the Antigene: 91 kDa
    Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA, immunoblotting and enzyme inhibition.

    Purification

    affinity purified

    Immunogen

    urease isolated and purified from Canavalia ensiformis (common jack-beans)

    Isotype

    IgG
  • Application Notes

    Recommended Dilution: 1 : 1000 - 1 : 30 000 for techniques listed above.

    Comment

    The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    For reconstitution please add 1 mL of sterile destilled water.

    Buffer

    phosphate buffer saline

    Handling Advice

    Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

    Storage

    -20 °C
  • Target

    Urease (URE)

    Alternative Name

    Urease

    Background

    Urease (EC=3.5.1.5) is an enzyme involved in nitrogen metabolism. It is catalyzes the hydrolysis of urea into carbon dioxide and ammonia.

    Molecular Weight

    91 kDa
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