Urease antibody
Quick Overview for Urease antibody (ABIN488562)
Target
Reactivity
Host
Clonality
Conjugate
Application
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Characteristics
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Expected / apparent Molecular Weight of the Antigene: 91 kDa
Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA, immunoblotting and enzyme inhibition. -
Purification
- affinity purified
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Immunogen
- urease isolated and purified from Canavalia ensiformis (common jack-beans)
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Isotype
- IgG
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Application Notes
- Recommended Dilution: 1 : 1000 - 1 : 30 000 for techniques listed above.
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Comment
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The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- For reconstitution please add 1 mL of sterile destilled water.
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Buffer
- phosphate buffer saline
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Handling Advice
- Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Storage
- -20 °C
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- Urease (URE)
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Alternative Name
- Urease
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Background
- Urease (EC=3.5.1.5) is an enzyme involved in nitrogen metabolism. It is catalyzes the hydrolysis of urea into carbon dioxide and ammonia.
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Molecular Weight
- 91 kDa
Target
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