Urease antibody (Biotin)

Catalog No. ABIN488563

This Rabbit Polyclonal antibody specifically detects Urease in WB, ELISA, IF and IHC. It exhibits reactivity toward Canavalia ensiformis.

Quick Overview for Urease antibody (Biotin) (ABIN488563)

Target: Urease (URE)

Reactivity: Canavalia ensiformis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Urease antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC)

Product Details anti-Urease Antibody (Biotin)

Characteristics: Expected / apparent Molecular Weight of the Antigene: 91 kDa
Antibody is labelled with biotin using N-hydroxysuccinimidobiotin. Antibody potency and purity has been evaluated by immunoelectrophoresis, single radialimmunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.

Purification: affinity purified

Immunogen: urease isolated and purified from Canavalia ensiformis (common jack-beans)

Isotype: IgG

Application Details

Application Notes: Recommended Dilution: 1 : 1000 - 1 : 30 000 for techniques listed above.

Comment:

Biotin/IgG protein molar ration is approximately 6.2. No foreign proteins are added.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution please add 1 mL of sterile destilled water.

Buffer: phosphate buffer saline

Handling Advice: Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Storage: -20 °C

Target Details for Urease

Target: Urease (URE)

Alternative Name: Urease

Background: Urease (EC=3.5.1.5) is an enzyme involved in nitrogen metabolism. It is catalyzes the hydrolysis of urea into carbon dioxide and ammonia.

Molecular Weight: 91 kDa