Urease antibody (Biotin)

Catalog No. ABIN488563

This Rabbit Polyclonal antibody specifically detects Urease in WB, ELISA, IF and IHC. It exhibits reactivity toward Canavalia ensiformis.

Quick Overview for Urease antibody (Biotin) (ABIN488563)

Target: Urease (URE)

Reactivity: Canavalia ensiformis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Urease antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC)

Product Details anti-Urease Antibody (Biotin)

Purpose: Anti-Urease, Biotin conjugated

Cross-Reactivity (Details): Canavalia ensiformis
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Predicted Reactivity: Arabidopsis thaliana,Glycine max,Ricinus communis

Purification: Purified IgG

Immunogen: urease isolated and purified from Canavalia ensiformis (common jack-beans)

Isotype: IgG

Application Details

Application Notes: 1 : 1000-1 : 30 000 (ELISA), (IF), (IHC), (WB)

Comment:

Biotin/IgG protein molar ration is approximately 6.2. No foreign proteins are added.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 1 mL of sterile destilled water

Buffer: Purified IgG in PBS.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details for Urease

Target: Urease (URE)

Alternative Name: Urease

Background:

Background: Urease (EC=3.5.1.5) is an enzyme involved in nitrogen metabolism. It is catalyzes the hydrolysis of urea into carbon dioxide and ammonia.

Additional information: Antibody is labelled with biotin using N-hydroxysuccinimidobiotin, Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition

Molecular Weight: 91 kDa