Urease antibody
Quick Overview for Urease antibody (ABIN488562)
Target
Reactivity
Host
Clonality
Conjugate
Application
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Purpose
- Anti-Urease
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Cross-Reactivity (Details)
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Canavalia ensiformis
Not reactive in: No confirmed exceptions from predicted reactivity are currently known -
Predicted Reactivity
- Arabidopsis thaliana,Glycine max,Ricinus communis
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Purification
- Purified IgG
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Immunogen
- urease isolated and purified from Canavalia ensiformis (common jack-beans)
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Isotype
- IgG
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Application Notes
- 1 : 1000-1 : 30 000 (ELISA), (IF), (IHC), (WB)
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Comment
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The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- For reconstitution add 1 mL of sterile destilled water
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Buffer
- Purified IgG in PBS.
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Handling Advice
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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Storage
- -20 °C
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Storage Comment
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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- Urease (URE)
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Alternative Name
- Urease
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Background
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Background: Urease (EC=3.5.1.5) is an enzyme involved in nitrogen metabolism. It is catalyzes the hydrolysis of urea into carbon dioxide and ammonia.
Additional information: Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition
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Molecular Weight
- 91 kDa
Target
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