Ricin antibody
Quick Overview for Ricin antibody (ABIN488640)
Target
Reactivity
Host
Clonality
Conjugate
Application
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Purpose
- Anti-Ricin (RTA subunit), clone MMA
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Cross-Reactivity (Details)
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Ricinus communis
Not reactive in: No confirmed exceptions from predicted reactivity are currently known -
Predicted Reactivity
- Ricinus communis
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Purification
- Purified IgG
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Immunogen
- Ricin
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Isotype
- IgG1
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Application Notes
- The optimal working dilution should be determined by the investigator, for specific application
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Comment
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Detected epitope is located on the surface of RTA subunit not accssible in intact ricin molecule. This antibody does not cross-react to whole ricin molecule.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- For reconstitution add 200 μL of sterile water
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Buffer
- Purified IgG in PBS pH 7.4 with mannitol.
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Handling Advice
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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Storage
- 4 °C,-80 °C
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Storage Comment
- Store at -70°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. , For storage at 4°C sodium azide can be added
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- Ricin
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Background
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Background: Ricin is a protein found in castor bean (Ricinus communis) acting as protein synthesis inhibitor. It is a toxin involved in plant defence. A chain of ricin can inactivate few thousands of ribosomes per minute. B chain facilitates the entry of A chain into the cell.
Additional information: Antibodies have been purified by affinity chromatography on Protein G from tissue culture supernatant and lyophilized from solution containing: mannitol 1 % , dextran 1 %, NaCl 100 mM, sodium phosphate 10 mM, pH 7.5. If required, mannitol can be removed either by dialysis or by chromatography using mini columns Sephadex G50 fine. The concentration can be measured by absorbance at 280 nm (1.35 for 1 mg/mL) or by Bradford methodRicin detection limit is 5 ng/mL, signal-to-noise ratio is 15-50 up to 100 ng/mLEpitope recognized by this antibody is located on the surface of RTA subunit and not accessible in intact ricin molecule. Suggested protocol can be found here.
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Molecular Weight
- 29.8 kDa
Target
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