ABAT antibody (AA 388-500)

Catalog No. ABIN4886408

The Rabbit Polyclonal anti-ABAT antibody has been validated for WB, IF, ICC and FACS. It is suitable to detect ABAT in samples from Human, Mouse and Rat.

Quick Overview for ABAT antibody (AA 388-500) (ABIN4886408)

Target: ABAT (4-Aminobutyrate Aminotransferase (ABAT))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ABAT antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-ABAT Antibody

Binding Specificity: AA 388-500

Purpose: Anti-ABAT Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-ABAT Antibody Picoband® (ABIN4886408). Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human ABAT recombinant protein (Position: K388-K500). Human ABAT shares 93.9% and 94.5% amino acid (aa) sequence identity with mouse and rat ABAT, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. De Biase D, Barra D, Simmaco M, John RA, Bossa F (Jan 1995). "Primary structure and tissue distribution of human 4-aminobutyrate aminotransferase". European Journal of Biochemistry / FEBS 227 (1-2): 476-80. 2. Medina-Kauwe LK, Tobin AJ, De Meirleir L, Jaeken J, Jakobs C, Nyhan WL, Gibson KM (Jun 1999). "4-Aminobutyrate aminotransferase (GABA-transaminase) deficiency".Journal of Inherited Metabolic Disease 22 (4): 414-27.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for ABAT

Target: ABAT (4-Aminobutyrate Aminotransferase (ABAT))

Alternative Name: ABAT

Background:

Synonyms: 4-aminobutyrate aminotransferase, mitochondrial,2.6.1.19, (S)-3-amino-2-methylpropionate transaminase,2.6.1.22,GABA aminotransferase,GABA-AT,Gamma-amino-N-butyrate transaminase,GABA transaminase,GABA-T,L-AIBAT,ABAT,GABAT,

Tissue Specificity: Liver > pancreas > brain > kidney > heart > placenta.

Background: 4-Aminobutyrate aminotransferase is a protein that in humans is encoded by the ABAT gene. ABAT is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5- phosphate. The protein sequence is over 95 % similar to the pig protein. GABA is estimated to be present in nearly one-third of humans ynapses. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractoryseizures, and EEG abnormalities. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene.

Sequence Similarities: Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC4 sub-subfamily.

Molecular Weight: 54 kDa

Gene ID: 18

UniProt: P80404

Pathways: Monocarboxylic Acid Catabolic Process