ACO2 antibody (C-Term)

Catalog No. ABIN4886417

The Rabbit Polyclonal anti-ACO2 antibody has been validated for WB. It is suitable to detect ACO2 in samples from Human, Rat and Mouse.

Quick Overview for ACO2 antibody (C-Term) (ABIN4886417)

Target: ACO2 (Aconitase 2, Mitochondrial (ACO2))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACO2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ACO2 Antibody

Binding Specificity: AA 561-596, C-Term

Purpose: Anti-Aconitase 2/ACO2 Antibody Picoband®

Sequence: TSQRLQLLEP FDKWDGKDLE DLQILIKVKG KCTTDH

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Aconitase 2/ACO2 Antibody Picoband® (ABIN4886417). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Aconitase 2, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. "Entrez Gene: Aconitase 2, mitochondrial". 2. Geurts van Kessel, A. H. M., Westerveld, A., de Groot, P. G., Meera Khan, P., Hagemeijer, A. Regional localization of the genes coding for human ACO2, ARSA, and NAGA on chromosome 22. Cytogenet. Cell Genet. 28: 169-172, 1980. 3. Mirel, D. B., Marder, K., Graziano, J., Freyer, G., Zhao, Q., Mayeux, R., Wilhelmsen, K. C. Characterization of the human mitochondrial aconitase gene (ACO2). Gene 213: 205-218, 1998.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for ACO2

Target: ACO2 (Aconitase 2, Mitochondrial (ACO2))

Alternative Name: ACO2

Background:

Synonyms: Aconitate hydratase, mitochondrial,Aconitase,4.2.1.3,Citrate hydro-lyase,ACO2,

Tissue Specificity: Highly enriched in the brain. Much weaker expression in heart, lung and muscle.

Background: Aconitase 2, mitochondrial is a protein that in humans is encoded by the ACO2 gene. The protein encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15 (PRSS15), also known as Lon protease, after oxidative modification.

Molecular Weight: 85 kDa

Gene ID: 50

UniProt: Q99798