Acsl3 antibody (AA 12-224)

Catalog No. ABIN4886419

This Rabbit Polyclonal antibody specifically detects Acsl3 in WB. It exhibits reactivity toward Human.

Quick Overview for Acsl3 antibody (AA 12-224) (ABIN4886419)

Target: Acsl3 (Acyl-CoA Synthetase Long-Chain Family Member 3 (Acsl3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Acsl3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Acsl3 Antibody

Binding Specificity: AA 12-224

Purpose: Anti-ACSL3 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-ACSL3 Antibody Picoband® (ABIN4886419). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ACSL3 recombinant protein (Position: M12-I224). Human ACSL3 shares 91.1% and 90.1% amino acid (aa) sequence identity with mouse and rat ACSL3, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
1. "Entrez Gene: ACSL3 acyl-CoA synthetase long-chain family member 3". 2. Bakken AM, Farstad M, Holmsen H (Feb 1991)."Identity between palmitoyl-CoA synthetase and arachidonoyl-CoA synthetase in human platelet?".The Biochemical Journal. 274 ( Pt 1) (1): 145-52. 3. Minekura H, Kang MJ, Inagaki Y, Suzuki H, Sato H, Fujino T, Yamamoto TT (Oct 2001). "Genomic organization and transcription units of the human acyl-CoA synthetase 3 gene". Gene. 278 (1-2): 185-192.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Acsl3

Target: Acsl3 (Acyl-CoA Synthetase Long-Chain Family Member 3 (Acsl3))

Alternative Name: ACSL3

Background:

Synonyms: Long-chain-fatty-acid--CoA ligase 3,6.2.1.3,Long-chain acyl-CoA synthetase 3,LACS 3,ACSL3,ACS3, FACL3, LACS3,

Tissue Specificity: Expressed in breast, ductal and invasive ductal carcinomas of the breast, sporadic colorectal adenomas and carcinomas (at protein level). Expressed in fetal brain. Expressed in lung, amygdala, eye, prostate, pancreatic and prostate cancers, head and neck tumors and embryonal tumor.

Background: Long-chain-fatty-acid-CoA ligase 3 is an enzyme that in humans is encoded by the ACSL3 gene. The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in brain, and preferentially utilizes myristate, arachidonate, and eicosapentaenoate as substrates. The amino acid sequence of this isozyme is 92 % identical to that of rat homolog. Two transcript variants encoding the same protein have been found for this gene.

Molecular Weight: 80 kDa

Gene ID: 2181

UniProt: O95573

Pathways: SARS-CoV-2 Protein Interactome