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ADA antibody (AA 135-363)

This anti-ADA antibody is a Rabbit Polyclonal antibody detecting ADA in WB, IHC and FACS. Suitable for Human, Mouse and Rat.
Catalog No. ABIN4886424

Quick Overview for ADA antibody (AA 135-363) (ABIN4886424)

Target

See all ADA Antibodies
ADA (Adenosine Deaminase (ADA))

Reactivity

  • 65
  • 34
  • 18
  • 14
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
Human, Mouse, Rat

Host

  • 75
  • 20
  • 1
Rabbit

Clonality

  • 77
  • 19
Polyclonal

Conjugate

  • 52
  • 8
  • 6
  • 6
  • 4
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This ADA antibody is un-conjugated

Application

  • 85
  • 42
  • 15
  • 14
  • 13
  • 13
  • 13
  • 11
  • 10
  • 5
  • 3
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
  • Binding Specificity

    • 15
    • 9
    • 8
    • 8
    • 6
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 135-363

    Purpose

    Anti-ADA Antibody Picoband®

    Cross-Reactivity (Details)

    No cross-reactivity with other proteins

    Characteristics

    Anti-ADA Antibody Picoband® (ABIN4886424). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Purification

    Immunogen affinity purified.

    Immunogen

    E. coli-derived human ADA recombinant protein (Position: Q135-L363). Human ADA shares 82.5% and 82.9% amino acid (aa) sequence identity with mouse and rat ADA, respectively.

    Isotype

    IgG
  • Application Notes

    Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
    Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
    Flow Cytometry(Fixed), 1-3 μg/1x106 cells, Human
    1. Blackburn MR (2003). "Too much of a good thing: adenosine overload in adenosine-deaminase-deficient mice". Trends in Pharmacological Sciences 24 (2): 66-70. 2. Cristalli G, Costanzi S, Lambertucci C, Lupidi G, Vittori S, Volpini R, Camaioni E (Mar 2001). "Adenosine deaminase: functional implications and different classes of inhibitors". Medicinal Research Reviews 21 (2): 105-128. 3. Wilson DK, Rudolph FB, Quiocho FA (May 1991). "Atomic structure of adenosine deaminase complexed with a transition-state analog: understanding catalysis and immunodeficiency mutations". Science 252(5010): 1278-1284.

    Comment

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    Concentration

    500 μg/mL

    Buffer

    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Avoid repeated freezing and thawing.

    Storage

    4 °C,-20 °C

    Storage Comment

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Target

    ADA (Adenosine Deaminase (ADA))

    Alternative Name

    ADA

    Background

    Synonyms: Adenosine deaminase,3.5.4.4,Adenosine aminohydrolase,ADA,ADA1,

    Tissue Specificity: Found in all tissues, occurs in large amounts in T-lymphocytes and, at the time of weaning, in gastrointestinal tissues.

    Background: Adenosine Deaminase (also known as adenosine aminohydrolase, or ADA) is an enzyme involved in purine metabolism. Primarily, ADA in humans is involved in the development and maintenance of the immune system. However, ADA association has also been observed with epithelial cell differentiation, neurotransmission, and gestation maintenance. It has also been proposed that ADA, in addition to adenosine breakdown, stimulates release of excitatory amino acids and is necessary to the coupling of A1 adenosine receptors and heterotrimeric G proteins. Adenosine deaminase deficiency leads to pulmonary fibrosis, suggesting that chronic exposure to high levels of adenosine can exacerbate inflammation responses rather than suppressing them. It has also been recognized that adenosine deaminase protein and activity is upregulated in mouse hearts that overexpress HIF-1 alpha, which in part explains the attenuated levels of adenosine in HIF-1 alpha expressing hearts during ischemic stress.

    Molecular Weight

    41 kDa

    Gene ID

    100

    UniProt

    P00813

    Pathways

    Regulation of G-Protein Coupled Receptor Protein Signaling, Ribonucleoside Biosynthetic Process
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