ALDH1A2 antibody (AA 1-110)

Catalog No. ABIN4886449

This Rabbit Polyclonal antibody specifically detects ALDH1A2 in WB, IHC and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ALDH1A2 antibody (AA 1-110) (ABIN4886449)

Target: ALDH1A2 (Aldehyde Dehydrogenase 1 Family, Member A2 (ALDH1A2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALDH1A2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-ALDH1A2 Antibody

Binding Specificity: AA 1-110

Purpose: Anti-ALDH1A2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-ALDH1A2 Antibody Picoband® (ABIN4886449). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ALDH1A2 recombinant protein (Position: M1-A110). Human ALDH1A2 shares 95.5% amino acid (aa) sequence identity with both mouse and rat ALDH1A2.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. "Entrez Gene: ALDH1A2 aldehyde dehydrogenase 1 family, member A2". 2. Ono Y, Fukuhara N, Yoshie O (Dec 1998). "TAL1 and LIM-Only Proteins Synergistically Induce Retinaldehyde Dehydrogenase 2 Expression in T-Cell Acute Lymphoblastic Leukemia by Acting as Cofactors for GATA3". Mol Cell Biol 18 (12): 6939-50.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for ALDH1A2

Target: ALDH1A2 (Aldehyde Dehydrogenase 1 Family, Member A2 (ALDH1A2))

Alternative Name: ALDH1A2

Background:

Synonyms: Retinal dehydrogenase 2,RALDH 2,RalDH2,1.2.1.36,Aldehyde dehydrogenase family 1 member A2,Retinaldehyde-specific dehydrogenase type 2,RALDH (II),ALDH1A2,RALDH2,

Tissue Specificity: Widely expressed. Expressed at higher level in thymus (medullary epithelial cells and monocyte-dendritic cells), pancreas, adrenal cortex and testis. Expressed at lower level in the spleen, fetal liver and lymph nodes. Isoform 2 and isoform 3 seem to be less frequently expressed than isoform 1, if at all.

Background: Aldehyde dehydrogenase 1 family, member A2, also known as ALDH1A2 or retinaldehyde dehydrogenase 2 (RALDH2), is an enzyme that in humans is encoded by the ALDH1A2 gene. This protein belongs to the aldehyde dehydrogenase family of proteins. The product of this gene is an enzyme that catalyzes the synthesis of retinoic acid (RA) from retinaldehyde. Retinoic acid, the active derivative of vitamin A (retinol), is a hormonal signaling molecule that functions in developing and adult tissues. The studies of a similar mouse gene suggest that this enzyme and the cytochrome CYP26A1, concurrently establish local embryonic retinoic acid levels which facilitate posterior organ development and prevent spina bifida. Four transcript variants encoding distinct isoforms have been identified for this gene.

Molecular Weight: 57 kDa

Gene ID: 8854

UniProt: O94788

Pathways: Retinoic Acid Receptor Signaling Pathway