ALDH3A2 antibody (AA 1-100)

Catalog No. ABIN4886451

This anti-ALDH3A2 antibody is a Rabbit Polyclonal antibody detecting ALDH3A2 in WB and IHC. Suitable for Human and Rat.

Quick Overview for ALDH3A2 antibody (AA 1-100) (ABIN4886451)

Target: ALDH3A2 (Aldehyde Dehydrogenase 3 Family, Member A2 (ALDH3A2))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALDH3A2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-ALDH3A2 Antibody

Binding Specificity: AA 1-100

Purpose: Anti-ALDH3A2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-ALDH3A2 Antibody Picoband® (ABIN4886451). Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ALDH3A2 recombinant protein (Position: M1-Q100). Human ALDH3A2 shares 78% amino acid (aa) sequence identity with both mouse and rat ALDH3A2.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Human, Rat
1. Kelson TL, Secor McVoy JR, Rizzo WB (1997). "Human liver fatty aldehyde dehydrogenase: microsomal localization, purification, and biochemical characterization". Biochim. Biophys. Acta 1335 (1-2): 99-110. 2. Rogers GR, Markova NG, De Laurenzi V, Rizzo WB, Compton JG (1997). "Genomic organization and expression of the human fatty aldehyde dehydrogenase gene (FALDH)". Genomics 39 (2): 127-35.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for ALDH3A2

Target: ALDH3A2 (Aldehyde Dehydrogenase 3 Family, Member A2 (ALDH3A2))

Alternative Name: ALDH3A2

Background:

Synonyms: Fatty aldehyde dehydrogenase,1.2.1.3,Aldehyde dehydrogenase 10,Aldehyde dehydrogenase family 3 member A2,Microsomal aldehyde dehydrogenase,ALDH3A2,ALDH10, FALDH,

Tissue Specificity: Widely expressed. Expressed at higher level in thymus (medullary epithelial cells and monocyte-dendritic cells), pancreas, adrenal cortex and testis. Expressed at lower level in the spleen, fetal liver and lymph nodes. Isoform 2 and isoform 3 seem to be less frequently expressed than isoform 1, if at all.

Background: Fatty aldehyde dehydrogenase (or Long-chain-aldehyde dehydrogenase) is an aldehyde dehydrogenase enzyme that in human is encoded in the ALDH3A2 gene on chromosome 17. ALDH3A2 catalyzes the oxidation of long-chain aliphatic aldehydes into fatty acids. It is known to act on a variety of both saturated and unsaturated aliphatic aldehydes between 6 to 24 carbons in length, as well as dihydrophytal, a 20-carbon branched chain aldehyde. It requires NAD+ as a co-factor. The encoded enzyme is responsible for conversion of the sphingosine 1-phosphate (S1P) degradation product hexadecenal to hexadecenoic acid. ALD3H2 is expressed in the human liver and has been found to localize the microsome fraction inside the cell.

Molecular Weight: 55 kDa

Gene ID: 224

UniProt: P51648