APOE antibody (AA 55-294)

Catalog No. ABIN4886470

This Rabbit Polyclonal antibody specifically detects APOE in WB, IHC, ICC and IF. It exhibits reactivity toward Mouse and Rat.

Quick Overview for APOE antibody (AA 55-294) (ABIN4886470)

Target: APOE (Apolipoprotein E (APOE))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APOE antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-APOE Antibody

Binding Specificity: AA 55-294

Purpose: Anti-Apolipoprotein E/APOE Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Apolipoprotein E/APOE Antibody Picoband® (ABIN4886470). Tested in IF, IHC, ICC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse Apolipoprotein E recombinant protein (Position: D55-Q294). Mouse Apolipoprotein E shares 75% and 94.6% amino acid (aa) sequence identity with human and rat Apolipoprotein E, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Mouse
Immunofluorescence, 5 μg/mL, Mouse, Rat
1. APOE ε variants increase risk of warfarin-related intracerebral hemorrhage.Falcone GJ, et al. Neurology, 2014 Sep 23. 2. Calabuig-Navarro MV, et al. Apolipoprotein E genotype has a modest impact on the postprandial plasma response to meals of varying fat composition in healthy men in a randomized controlled trial. J Nutr, 2014 Nov.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for APOE

Target: APOE (Apolipoprotein E (APOE))

Alternative Name: Apoe

Background:

Synonyms: Apolipoprotein E,Apo-E,Apoe,

Tissue Specificity: Secreted in plasma.

Background: APOE is also known as AD2 or LPG. The protein encoded by this gene is a major apoprotein of the chylomicron. It binds to a specific liver and peripheral cell receptor, and is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. This gene maps to chromosome 19 in a cluster with the related apolipoprotein C1 and C2 genes. Mutations in this gene result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. Alternative splicing results in multiple transcript variants.

Molecular Weight: 36 kDa

Gene ID: 11816

UniProt: P08226

Pathways: Regulation of Cell Size, Lipid Metabolism