BCR antibody (AA 1-100)

Catalog No. ABIN4886483

The Rabbit Polyclonal anti-BCR antibody has been validated for WB, IF, FACS and ICC. It is suitable to detect BCR in samples from Human, Mouse and Rat.

Quick Overview for BCR antibody (AA 1-100) (ABIN4886483)

Target: BCR (Breakpoint Cluster Region (BCR))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BCR antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-BCR Antibody

Binding Specificity: AA 1-100

Purpose: Anti-Bcr Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Bcr Antibody Picoband® (ABIN4886483). Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Bcr recombinant protein (Position: M1-A100). Human Bcr shares 94% amino acid (aa) sequence identity with mouse Bcr.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. "Entrez Gene: Breakpoint cluster region". 2. Puil L, Liu J, Gish G, Mbamalu G, Bowtell D, Pelicci PG, Arlinghaus R, Pawson T (February 1994). "Bcr-Abl oncoproteins bind ly to activators of the Ras signalling pathway". EMBO J. 13 (4): 764-73. 3. Pendergast AM, Muller AJ, Havlik MH, Maru Y, Witte ON (July 1991). "BCR sequences essential for transformation by the BCR-ABL oncogene bind to the ABL SH2 regulatory domain in a non-phosphotyrosine-dependent manner".Cell 66 (1): 161-71.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for BCR

Target: BCR (Breakpoint Cluster Region (BCR))

Alternative Name: BCR

Background:

Synonyms: Breakpoint cluster region protein,2.7.11.1,Renal carcinoma antigen NY-REN-26,BCR,BCR1, D22S11,

Tissue Specificity: Ubiquitous, with higher levels in heart, substantia nigra, and kidney. .

Background: The breakpoint cluster region protein (BCR) is a protein that in humans is encoded by the BCR gene. A reciprocal translocation between chromosomes 22 and 9 produces the Philadelphia chromosome, which is often found in patients with chronic myelogenous leukemia. The chromosome 22 breakpoint for this translocation is located within the BCR gene. The translocation produces a fusion protein which is encoded by sequence from both BCR and ABL, the gene at the chromosome 9 breakpoint. Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for p21rac. Two transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 143 kDa

Gene ID: 613

UniProt: P11274

Pathways: Regulation of Leukocyte Mediated Immunity, Platelet-derived growth Factor Receptor Signaling