Osteocalcin antibody (AA 50-99)

Catalog No. ABIN4886487

This anti-Osteocalcin antibody is a Rabbit Polyclonal antibody detecting Osteocalcin in IHC and FACS. Suitable for Rat. This Primary Antibody has been cited in 7+ publications.

Quick Overview for Osteocalcin antibody (AA 50-99) (ABIN4886487)

Target: Osteocalcin (BGLAP)

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Osteocalcin antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-Osteocalcin Antibody

Binding Specificity: AA 50-99

Purpose: Anti-Osteocalcin/BGLAP Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Osteocalcin/BGLAP Antibody Picoband® (ABIN4886487). Tested in Flow Cytometry, IHC applications. This antibody reacts with Rat.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived rat Osteocalcin recombinant protein (Position: Y50-V99). Rat Osteocalcin shares 80.9% and 68.9% amino acid (aa) sequence identity with human and mouse Osteocalcin, respectively.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Rat1. Cancela L, Hsieh CL, Francke U, Price PA (September 1990). "Molecular structure, chromosome assignment, and promoter organization of the human matrix Gla protein gene". J. Biol. Chem. 265 (25): 15040-8. 2. Lee NK, Sowa H, Hinoi E, Ferron M, Ahn JD, Confavreux C, Dacquin R, Mee PJ, McKee MD, Jung DY, Zhang Z, Kim JK, Mauvais-Jarvis F, Ducy P, Karsenty G (August 2007). "Endocrine regulation of energy metabolism by the skeleton". Cell 130 (3): 456-69. 3. Puchacz E, Lian JB, Stein GS, Wozney J, Huebner K, Croce C (May 1989). "Chromosomal localization of the human osteocalcin gene". Endocrinology 124 (5): 2648-50.

Comment:

Antibody can be supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-Osteocalcin antibody (ABIN4886487)

Song, Wang, Zeng, Wang: "Synergistic effects of fibroblast growth factor-2 and bone morphogenetic protein-2 on bone induction." in: Molecular medicine reports, Vol. 16, Issue 4, pp. 4483-4492, (2018) (PubMed).

Liu, Ma, Wang, Zhu, Yang, Mei: "Demineralized bone matrix used for direct pulp capping in rats." in: PLoS ONE, Vol. 12, Issue 3, pp. e0172693, (2017) (PubMed).

Yang, Baban, Isales, Shi: "Role of glucocorticoid-induced leucine zipper (GILZ) in inflammatory bone loss." in: PLoS ONE, Vol. 12, Issue 8, pp. e0181133, (2017) (PubMed).

Che, Guo, Li, Wang, Wei: "Intramuscular injection of bone marrow mononuclear cells contributes to bone repair following midpalatal expansion in rats." in: Molecular medicine reports, Vol. 13, Issue 1, pp. 681-8, (2016) (PubMed).

Yin, Cheng, Qin, Yu, Yu, Zhong, Sun, Zhang: "Effects of Naringin on Proliferation and Osteogenic Differentiation of Human Periodontal Ligament Stem Cells In Vitro and In Vivo." in: Stem cells international, Vol. 2015, pp. 758706, (2015) (PubMed).

Cheng, Wang, Zhang, Shi: "The osteogenic potential of mesoporous bioglasses/silk and non-mesoporous bioglasses/silk scaffolds in ovariectomized rats: in vitro and in vivo evaluation." in: PLoS ONE, Vol. 8, Issue 11, pp. e81014, (2014) (PubMed).

Chen, Bai, Liao, Peng, Wu, Wang, Zeng, Xie: "Electrospun poly(L-lactide)/poly(?-caprolactone) blend nanofibrous scaffold: characterization and biocompatibility with human adipose-derived stem cells." in: PLoS ONE, Vol. 8, Issue 8, pp. e71265, (2013) (PubMed).

Target Details for Osteocalcin

Target: Osteocalcin (BGLAP)

Alternative Name: Bglap

Background:

Synonyms: Osteocalcin,Bone Gla protein,BGP,Gamma-carboxyglutamic acid-containing protein,Bglap,Bglap2,

Tissue Specificity: Bone.

Background: Osteocalcin, also known as bone gamma-carboxyglutamic acid-containing protein (BGLAP), is a noncollagenous protein found in bone and dentin. In humans, the osteocalcin is encoded by the BGLAP gene. Its receptor is GPRC6A. It is mapped to 1q22. Osteocalcin may play a role in the body's metabolic regulation and is pro-osteoblastic, or bone-building. It acts as a hormone in the body, causing beta cellsin the pancreas to release more insulin, and at the same time ing fat cells to release the hormone adiponectin, which increases sensitivity to insulin. Also, it may play a role in male fertility. And it is found that picomolar amounts of osteocalcin affected insulin secretion and beta-cell proliferation.

Molecular Weight: 29 kDa, 60 kDa

Gene ID: 25295

UniProt: P04640