CAP1 antibody (AA 2-123)

Catalog No. ABIN4886499

This anti-CAP1 antibody is a Rabbit Polyclonal antibody detecting CAP1 in WB. Suitable for Human, Mouse and Rat.

Quick Overview for CAP1 antibody (AA 2-123) (ABIN4886499)

Target: CAP1 (CAP, Adenylate Cyclase-Associated Protein 1 (CAP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CAP1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CAP1 Antibody

Binding Specificity: AA 2-123

Purpose: Anti-CAP1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-CAP1 Antibody Picoband® (ABIN4886499). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human CAP1 recombinant protein (Position: A2-R123). Human CAP1 shares 92.6% and 93.4% amino acid (aa) sequence identity with mouse and rat CAP1, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. "Entrez Gene: CAP1 CAP, adenylate cyclase-associated protein 1 (yeast)". 2. Hubberstey A, Yu G, Loewith R, Lakusta C, Young D (Apr 1997). "Mammalian CAP interacts with CAP, CAP2, and actin". J Cell Biochem 61 (3): 459-66. 3. Matviw H, Yu G, Young D (Nov 1992). "Identification of a human cDNA encoding a protein that is structurally and functionally related to the yeast adenylyl cyclase-associated CAP proteins". Mol Cell Biol 12 (11): 5033-40.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for CAP1

Target: CAP1 (CAP, Adenylate Cyclase-Associated Protein 1 (CAP1))

Alternative Name: CAP1

Background:

Synonyms: Adenylyl cyclase-associated protein 1,CAP 1,CAP1,CAP,

Tissue Specificity: High-molecular-weight caldesmon (isoform 1) is predominantly expressed in smooth muscles, whereas low-molecular- weight caldesmon (isoforms 2, 3, 4 and 5) are widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart.

Background: Adenylyl cyclase-associated protein 1 is an enzyme that in humans is encoded by the CAP1 gene. This gene is mapped to 1p34.2. The protein encoded by this gene is related to the S. cerevisiae CAP protein, which is involved in the cyclic AMP pathway. And the human protein is able to interact with other molecules of the same protein, as well as with CAP2 and actin. Alternatively spliced transcript variants have been identified.

Molecular Weight: 60 kDa

Gene ID: 10487

UniProt: Q01518

Pathways: cAMP Metabolic Process