CCT8 antibody (AA 421-548)

Catalog No. ABIN4886516

The Rabbit Polyclonal anti-CCT8 antibody has been validated for WB, IHC, IF, FACS, ICC and IHC (fro). It is suitable to detect CCT8 in samples from Human, Mouse and Rat.

Quick Overview for CCT8 antibody (AA 421-548) (ABIN4886516)

Target: CCT8 (Chaperonin Containing TCP1, Subunit 8 (Theta) (CCT8))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCT8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-CCT8 Antibody

Binding Specificity: AA 421-548

Purpose: Anti-TCP1 theta/CCT8 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-TCP1 theta/CCT8 Antibody Picoband® (ABIN4886516). Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CCT8 recombinant protein (Position: K421-D548). Human CCT8 shares 98.4% amino acid (aa) sequence identity with mouse CCT8.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human1. "Entrez Gene: CCT8 chaperonin containing TCP1, subunit 8 (theta)". 2. Kubota H, Hynes G, Willison K (Apr 1995). "The eighth Cct gene, Cctq, encoding the theta subunit of the cytosolic chaperonin containing TCP-1". Gene 154 (2): 231-6.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for CCT8

Target: CCT8 (Chaperonin Containing TCP1, Subunit 8 (Theta) (CCT8))

Alternative Name: CCT8

Background:

Synonyms: T-complex protein 1 subunit theta,TCP-1-theta,CCT-theta,Renal carcinoma antigen NY-REN-15,CCT8,C21orf112, CCTQ, KIAA0002,

Tissue Specificity: Ubiquitously expressed.

Background: T-complex protein 1 subunit theta is a protein that in humans is encoded by the CCT8 gene. This gene encodes the theta subunit of the CCT chaperonin, which is abundant in the eukaryotic cytosol and may be involved in the transport and assembly of newly synthesized proteins. Alternative splicing results in multiple transcript variants of this gene. A pseudogene related to this gene is located on chromosome 1.

Molecular Weight: 60 kDa

Gene ID: 10694

UniProt: P50990