Complement Factor B antibody (AA 518-764)

Catalog No. ABIN4886536

This Rabbit Polyclonal antibody specifically detects Complement Factor B in WB and IHC. It exhibits reactivity toward Human.

Quick Overview for Complement Factor B antibody (AA 518-764) (ABIN4886536)

Target: Complement Factor B (CFB)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Complement Factor B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Complement Factor B Antibody

Binding Specificity: AA 518-764

Purpose: Anti-Factor B/CFB Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Factor B/CFB Antibody Picoband® (ABIN4886536). Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human Factor B recombinant protein (Position: E518-L764). Human Factor B shares 84.2% amino acid (aa) sequence identity with mouse Factor B.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Human
1. "Entrez Gene: CFB complement factor B". 2. Benkmann, H. G., Goedde, H. W., Agarwal, D. P., Flatz, G., Rahimi, A., Kaifie, R. S., Delbruck, H. Properdin factor B polymorphism in Afghanistan. Hum. Hered. 30: 39-43, 1980. 3. Campbell, R. D., Porter, R. R. Molecular cloning and characterization of the gene coding for human complement protein factor B.Proc. Nat. Acad. Sci. 80: 4464-4468, 1983.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Complement Factor B

Target: Complement Factor B (CFB)

Alternative Name: CFB

Background:

Synonyms: Complement factor B,3.4.21.47,C3/C5 convertase,Glycine-rich beta glycoprotein,GBG,PBF2,Properdin factor B,Complement factor B Ba fragment,Complement factor B Bb fragment,CFB,BF, BFD,

Tissue Specificity: Highly expressed in mitotic cells with low expression during interphase. Detected at highest levels in skeletal muscle and colon with lower levels in kidney, small intestine, placenta and lung. Up-regulated in a number of human tumor cell lines, as well as in colorectal cancer, breast carcinoma and lung cancer. Also expressed in cells with phenotypes reminiscent of mesenchymal stem cells and neural stem cells. .

Background: Complement factor B is a protein that in humans is encoded by the CFB gene. This gene encodes complement factor B, a component of the alternative pathway of complement activation. Factor B circulates in the blood as a single chain polypeptide. Upon activation of the alternative pathway, it is cleaved by complement factor D yielding the noncatalytic chain Ba and the catalytic subunit Bb. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. This cluster includes several genes involved in regulation of the immune reaction. Polymorphisms in this gene are associated with a reduced risk of age-related macular degeneration. The polyadenylation site of this gene is 421 bp from the 5' end of the gene for complement component 2.

Molecular Weight: 86 kDa

Gene ID: 629

UniProt: P00751

Pathways: Complement System, Proton Transport, Ribonucleoside Biosynthetic Process