CPM antibody (C-Term)

Catalog No. ABIN4886547

This anti-CPM antibody is a Rabbit Polyclonal antibody detecting CPM in WB and FACS. Suitable for Human.

Quick Overview for CPM antibody (C-Term) (ABIN4886547)

Target: CPM (Carboxypeptidase M (CPM))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CPM antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-CPM Antibody

Binding Specificity: AA 286-316, C-Term

Purpose: Anti-Carboxypeptidase M/CPM Antibody Picoband®

Sequence: KYPREEKLPS FWNNNKASLI EYIKQVHLGV K

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Carboxypeptidase M/CPM Antibody Picoband® (ABIN4886547). Tested in Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human CPM, different from the related mouse sequence by two amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Kas, K., Schoenmakers, E. F. P. M., Van de Ven, W. J. M. Physical map location of the human carboxypeptidase M gene (CPM) distal to D12S375 and proximal to D12S8 at chromosome 12q15. Genomics 30: 403-405, 1995. 2. Rehli, M., Krause, S. W., Kreutz, M., Andreesen, R. Carboxypeptidase M is identical to the MAX.1 antigen and its expression is associated with monocyte to macrophage differentiation. J. Biol. Chem. 270: 15644-15649, 1995. 3. Tan, F., Chan, S. J., Steiner, D. F., Schilling, J. W., Skidgel, R. A. Molecular cloning and sequencing of the cDNA for human membrane-bound carboxypeptidase M: comparison with carboxypeptidases A, B, H, and N. J. Biol. Chem. 264: 13165-13170, 1989.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for CPM

Target: CPM (Carboxypeptidase M (CPM))

Alternative Name: CPM

Background:

Synonyms: Carboxypeptidase M,CPM,3.4.17.12 ,CPM,

Tissue Specificity: Four different transcripts were found to be expressed in a distinct tissue distribution.

Background: CPM, mapped to 12q15, is known as Carboxypeptidase M. The protein encoded by this gene is a membrane-bound arginine/lysine carboxypeptidase. Its expression is associated with monocyte to macrophage differentiation. This encoded protein contains hydrophobic regions at the amino and carboxy termini and has 6 potential asparagine-linked glycosylation sites. The active site residues of carboxypeptidases A and B are conserved in this protein. Three alternatively spliced transcript variants encoding the same protein have been described for this gene.

Molecular Weight: 65 kDa

Gene ID: 1368

UniProt: P14384