Doublecortin antibody (N-Term)

Catalog No. ABIN4886559

This anti-Doublecortin antibody is a Rabbit Polyclonal antibody detecting Doublecortin in WB. Suitable for Human, Mouse and Rat. This Primary Antibody has been cited in 1 publication.

Quick Overview for Doublecortin antibody (N-Term) (ABIN4886559)

Target: Doublecortin (DCX)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Doublecortin antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Doublecortin Antibody

Binding Specificity: AA 74-100, N-Term

Purpose: Anti-Doublecortin/DCX Antibody Picoband®

Sequence: QSLRFHQNME LDFGHFDERD KTSRNMR

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Doublecortin/DCX Antibody Picoband® (ABIN4886559). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Doublecortin.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse, Rat, Human
1. Brown JP, Couillard-Després S, Cooper-Kuhn CM, Winkler J, Aigner L, Kuhn HG (December 2003). "Transient expression of doublecortin during adult neurogenesis". J. Comp. Neurol. 467 (1): 1-10. 2. Couillard-Despres S, Winner B, Schaubeck S, Aigner R, Vroemen M, Weidner N, Bogdahn U, Winkler J, Kuhn HG, Aigner L (January 2005). "Doublecortin expression levels in adult brain reflect neurogenesis".Eur. J. Neurosci. 21 (1): 1-14.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-Doublecortin antibody (ABIN4886559)

Zhang, Du, Yang, Wang, Yu, Huang, Hu, Liu, Fu: "Granulocyte colony-stimulating factor increases the therapeutic efficacy of bone marrow mononuclear cell transplantation in cerebral ischemia in mice." in: BMC neuroscience, Vol. 12, pp. 61, (2011) (PubMed).

Target Details for Doublecortin

Target: Doublecortin (DCX)

Alternative Name: DCX

Background:

Synonyms: Neuronal migration protein doublecortin,Doublin,Lissencephalin-X,Lis-X,DCX,DBCN, LISX,

Tissue Specificity: Highly expressed in neuronal cells of fetal brain (in the majority of cells of the cortical plate, intermediate zone and ventricular zone), but not expressed in other fetal tissues. In the adult, highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas.

Background: Neuronal migration protein doublecortin, also known as doublin or lissencephalin-X, is a protein that in humans is encoded by the DCX gene. This gene encodes a member of the doublecortin family. The protein encoded by this gene is a cytoplasmic protein and contains two doublecortin domains, which bind microtubules. In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The encoded protein appears to neuronal migration by regulating the organization and stability of microtubules. In addition, the encoded protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in this gene cause abnormal migration of neurons during development and disrupt the layering of the cortex, leading to epilepsy, mental retardation, subcortical band heterotopia ("double cortex" syndrome) in females and lissencephaly ("smooth brain" syndrome) in males. Multiple transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 40 kDa

Gene ID: 1641

UniProt: O43602