GIP antibody (AA 44-85)

Catalog No. ABIN4886603

The Rabbit Polyclonal anti-GIP antibody has been validated for WB and IHC. It is suitable to detect GIP in samples from Rat and Mouse.

Quick Overview for GIP antibody (AA 44-85) (ABIN4886603)

Target: GIP (Gastric Inhibitory Polypeptide (GIP))

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GIP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-GIP Antibody

Binding Specificity: AA 44-85

Purpose: Anti-GIP Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-GIP Antibody Picoband® (ABIN4886603). Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived mouse GIP recombinant protein (Position: Y44-Q85). Mouse GIP shares 92.9% amino acid (aa) sequence identity with both human and rat GIP.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse
Immunohistochemistry, 2-5 μg/mL, Mouse, Rat
1. Gaudin-Audrain C, Irwin N, Mansur S, Flatt PR, Thorens B, Baslé M, Chappard D, Mabilleau G (Mar 2013). "Glucose-dependent insulinotropic polypeptide receptor deficiency leads to modifications of trabecular bone volume and quality in mice". Bone 53 (1): 221-30. 2. Mieczkowska A, Irwin N, Flatt PR, Chappard D, Mabilleau G (Oct 2013). "Glucose-dependent insulinotropic polypeptide (GIP) receptor deletion leads to reduced bone strength and quality".Bone 56 (2): 337-42.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for GIP

Target: GIP (Gastric Inhibitory Polypeptide (GIP))

Alternative Name: Gip

Background:

Synonyms: Gastric inhibitory polypeptide,GIP,Glucose-dependent insulinotropic polypeptide,Gip,

Tissue Specificity: Predominantly expressed in kidney and brain. Detected at much lower levels in heart and skeletal muscle. .

Background: Gastric inhibitory polypeptide (GIP), also known as the glucose-dependent insulinotropic peptide, is an inhibiting hormone of the secretin family of hormones. GIP is thought to have significant effects on fatty acid metabolism through stimulation of lipoprotein lipase activity in adipocytes. Additionally, GIP release has been demonstrated in the ruminant animal and may play a role in nutrient partitioning in milk production (lipid metabolism). Recently, GIP appeared as a major player in bone remodelling. It was evidenced that genetic ablation of the GIP receptor in mice resulted in profound alterations of bone microarchitecture through modification of the adipokine network. Furthermore, the deficiency in GIP receptors has also been associated in mice with a dramatic decrease in bone quality and a subsequent increase in fracture risk.

Molecular Weight: 18 kDa

Gene ID: 14607

UniProt: P48756

Pathways: Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Regulation of Lipid Metabolism by PPARalpha, Lipid Metabolism