GLRX3 antibody (AA 89-177)

Catalog No. ABIN4886606

The Rabbit Polyclonal anti-GLRX3 antibody has been validated for WB. It is suitable to detect GLRX3 in samples from Human, Mouse and Rat.

Quick Overview for GLRX3 antibody (AA 89-177) (ABIN4886606)

Target: GLRX3 (Glutaredoxin 3 (GLRX3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLRX3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GLRX3 Antibody

Binding Specificity: AA 89-177

Purpose: Anti-TXNL2/GLRX3 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-TXNL2/GLRX3 Antibody Picoband® (ABIN4886606). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human TXNL2 recombinant protein (Position: L89-S177). Human TXNL2 shares 93.3% and 94.4% amino acid (aa) sequence identity with mouse and rat TXNL2, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse, Rat, Human
1. Stanchi F, Bertocco E, Toppo S, Dioguardi R, Simionati B, Cannata N, Zimbello R, Lanfranchi G, Valle G (Jan 2001). "Characterization of 16 novel human genes showing high similarity to yeast sequences". Yeast 18 (1): 69-80. 2. Witte S, Villalba M, Bi K, Liu Y, Isakov N, Altman A (Feb 2000). "Inhibition of the c-Jun N-terminal kinase/AP-1 and NF-kappaB pathways by PICOT, a novel protein kinase C-interacting protein with a thioredoxin homology domain". J Biol Chem 275 (3): 1902-9.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for GLRX3

Target: GLRX3 (Glutaredoxin 3 (GLRX3))

Alternative Name: GLRX3

Background:

Synonyms: Glutaredoxin-3,PKC-interacting cousin of thioredoxin,PICOT,PKC-theta-interacting protein,PKCq-interacting protein,Thioredoxin-like protein 2,GLRX3,PICOT, TXNL2,HUSSY-22,

Tissue Specificity: Expressed in heart, spleen, testis and, to a lower extent, in thymus and peripheral blood leukocytes. Weakly expressed in lung, placenta, colon and small intestine.

Background: Glutaredoxin-3 is a protein that in humans is encoded by the GLRX3 gene. This gene encodes a member of the glutaredoxin family. Glutaredoxins are oxidoreductase enzymes that reduce a variety of substrates using glutathione as a cofactor. The encoded protein binds to and modulates the function of protein kinase C theta. The encoded protein may also inhibit apoptosis and play a role in cellular growth, and the expression of this gene may be a marker for cancer. Pseudogenes of this gene are located on the short arm of chromosomes 6 and 9. Alternatively spliced transcript variants have been observed for this gene.

Molecular Weight: 37 kDa

Gene ID: 10539

UniProt: O76003

Pathways: Cell RedoxHomeostasis