HDGF antibody (C-Term)

Catalog No. ABIN4886613

Product Details anti-HDGF Antibody

Target: HDGF (Hepatoma-Derived Growth Factor (HDGF))

Binding Specificity: AA 61-97, C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HDGF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Purpose: Anti-HDGF Antibody Picoband®

Sequence: KDLFPYEESK EKFGKPNKRK GFSEGLWEIE NNPTVKA

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-HDGF Antibody Picoband® (ABIN4886613). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human HDGF, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human1. "Entrez Gene: HDGF hepatoma-derived growth factor (high-mobility group protein 1-like)". 2. Everett AD, Bushweller J (2003). "Hepatoma derived growth factor is a nuclear targeted mitogen.". Current drug targets. 4 (5): 367-71. 3. Wanschura S, Schoenmakers EF, Huysmans C, Bartnitzke S, Van de Ven WJ, Bullerdiek J (May 1997). "Mapping of the gene encoding the human hepatoma-derived growth factor (HDGF) with homology to the high-mobility group (HMG)-1 protein to Xq25". Genomics. 32 (2): 298-300.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for HDGF

Target: HDGF (Hepatoma-Derived Growth Factor (HDGF))

Alternative Name: HDGF (HDGF Products)

Background:

Synonyms: Hepatoma-derived growth factor,HDGF,High mobility group protein 1-like 2,HMG-1L2,HDGF,HMG1L2,

Tissue Specificity: Ubiquitous.

Background: Hepatoma-derived growth factor (HDGF), also known as high mobility group protein 1-like 2 (HMG-1L2), is a protein that in humans is encoded by the HDGF gene. This gene encodes a member of the hepatoma-derived growth factor family. The encoded protein has mitogenic and DNA-binding activity and may play a role in cellular proliferation and differentiation. High levels of expression of this gene enhance the growth of many tumors. And this gene was thought initially to be located on chromosome X, however, that location has been determined to correspond to a related pseudogene. Alternatively spliced transcript variants encoding distinct isoforms have been described.

Molecular Weight: 38 kDa

Gene ID: 3068

UniProt: P51858

Pathways: ER-Nucleus Signaling