INA antibody (AA 71-161)

Catalog No. ABIN4886634

This anti-INA antibody is a Rabbit Polyclonal antibody detecting INA in WB, IHC, IF, ICC and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for INA antibody (AA 71-161) (ABIN4886634)

Target: INA (Internexin Neuronal Intermediate Filament Protein, alpha (INA))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This INA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-INA Antibody

Binding Specificity: AA 71-161

Purpose: Anti-Alpha Internexin/INA Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Alpha Internexin/INA Antibody Picoband® (ABIN4886634). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Alpha Internexin recombinant protein (Position: A71-R161). Human Alpha Internexin shares 98.9% amino acid (aa) sequence identity with both mouse and rat Alpha Internexin.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Mouse, Rat
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human
1. Chan, S.-O., Chiu, F.-C. Cloning and developmental expression of human 66 kd neurofilament protein. Molec. Brain Res. 29: 177-184, 1995. 2. Levavasseur F, Zhu Q, and JP Julien. No requirement of alpha-internexin for nervous system development and for radial growth of axons. Molecular Brain Research. 69:104-112. (1999).

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for INA

Target: INA (Internexin Neuronal Intermediate Filament Protein, alpha (INA))

Alternative Name: INA

Background:

Synonyms: Alpha-internexin,Alpha-Inx,66 kDa neurofilament protein,NF-66,Neurofilament-66,Neurofilament 5,INA,NEF5,

Tissue Specificity: Found predominantly in adult CNS.

Background: Alpha-Internexin (INA, also NF-66) is a 66 kDa member of the intermediate filament (IF) protein family. The protein was originally purified from rat optic nerve and spinal cord. And the protein copurifies with other neurofilament subunits, as it was originally discovered, however in some mature neurons it can be the only neurofilament expressed. The protein is present in developing neuroblasts and in the Central Nervous System of adults. Meanwhile, the protein is a major component of the intermediate filament network in small interneurons and cerebellar granule cells, where it is present in the parallel fibers.

Sequence Similarities: Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC4 sub-subfamily.

Molecular Weight: 62-66 kDa

Gene ID: 9118

UniProt: Q16352