ISG15 antibody (AA 2-155)

Catalog No. ABIN4886639

The Rabbit Polyclonal anti-ISG15 antibody has been validated for WB and IHC. It is suitable to detect ISG15 in samples from Mouse.

Quick Overview for ISG15 antibody (AA 2-155) (ABIN4886639)

Target: ISG15 (ISG15 Ubiquitin-Like Modifier (ISG15))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ISG15 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-ISG15 Antibody

Binding Specificity: AA 2-155

Purpose: Anti-ISG15 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-ISG15 Antibody Picoband® (ABIN4886639). Tested in IHC, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse ISG15 recombinant protein (Position: A2-G155). Mouse ISG15 shares 65.8% amino acid (aa) sequence identity with human ISG15.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse
Western blot, 0.1-0.5 μg/mL, Mouse
1. "Entrez Gene: ISG15 ISG15 ubiquitin-like modifier". 2. Blomstrom DC, Fahey D, Kutny R, Korant BD, Knight E Jr (Aug 1986). "Molecular characterization of the interferon-induced 15- kDa protein. Molecular cloning and nucleotide and amino acid sequence". J Biol Chem261 (19): 8811-6. 3. Morales, David J., Lenschow, Deborah J. (December 2013). "The Antiviral Activities of ISG15".Journal of Molecular Biology 425 (24): 4995-5008.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for ISG15

Target: ISG15 (ISG15 Ubiquitin-Like Modifier (ISG15))

Alternative Name: Isg15

Background:

Synonyms: Ubiquitin-like protein ISG15,Interferon-induced 15 kDa protein,Interferon-induced 17 kDa protein,IP17,Ubiquitin cross-reactive protein,Isg15,G1p2, Ucrp,

Background: Interferon-stimulated gene 15 (ISG15) is a 17 kDA secreted protein that in humans is encoded by the ISG15 gene. The protein encoded by this gene is a ubiquitin-like protein that is conjugated to intracellular target proteins upon activation by interferon-alpha and interferon-beta. Several functions have been ascribed to the encoded protein, including chemotactic activity towards neutrophils, ion of ligated target proteins to intermediate filaments, cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this protein have been found to be noncovalently attached to intermediate filaments, this protein is sometimes secreted.

Molecular Weight: 15 kDa

Gene ID: 100038882

UniProt: Q64339