CD11b antibody (AA 260-360)

Catalog No. ABIN4886642

There are 4+ publications for this product available. The Rabbit Polyclonal anti-CD11b antibody is suitable to detect CD11b in samples from Mouse. It has been validated for WB.

Quick Overview for CD11b antibody (AA 260-360) (ABIN4886642)

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD11b antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CD11b Antibody

Binding Specificity: AA 260-360

Purpose: Anti-CD11b/ITGAM Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: coli-derived mouse CD11b recombinant protein (Position: E260-T360). Mouse CD11b shares 82.2% amino acid (aa) sequence identity with human CD11b.

Characteristics: Anti-CD11b/ITGAM Antibody. Tested in WB applications. This antibody reacts with Mouse.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived mouse CD11b recombinant protein (Position: E260-T360). Mouse CD11b shares 82.2% amino acid (aa) sequence identity with human CD11b.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-CD11b antibody (ABIN4886642)

Mu, Jing, Guo: "Expressions of CD11a, CD11b, and CD11c integrin proteins in rats with myocardial hypertrophy." in: Iranian journal of basic medical sciences, Vol. 17, Issue 11, pp. 874-8, (2015) (PubMed).

Yuan, Su, Pu, Liu, Chen, Zhu, Zhu, Zhang, He: "Ethyl pyruvate promotes spinal cord repair by ameliorating the glial microenvironment." in: British journal of pharmacology, Vol. 166, Issue 2, pp. 749-63, (2012) (PubMed).

Leng, Zhan, Wang, Liu, Gong, Gao, Wu, Wang, Zhao, Wang, Zhang, Pang, Qian: "Anti-heat shock protein 70 autoantibody epitope changes and BD091 promotes atherosclerosis in rats." in: Cell stress & chaperones, Vol. 15, Issue 6, pp. 947-58, (2011) (PubMed).

Shi, Xun, Yue, Zhang, Ren, Wang, Wu, Shi, Yang, Lei: "Cloning, characterization, and expression analysis of goat (Capra hircus) phospholipid hydroperoxide glutathione peroxidase (PHGPx)." in: International journal of biological sciences, Vol. 6, Issue 4, pp. 316-26, (2010) (PubMed).

Target Details for CD11b

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Alternative Name: Itgam

Background:

Background: Integrin alpha M (ITGAM) is one protein subunit that forms the heterodimeric integrin alpha-M beta-2 (αMβ2) molecule, also known as macrophage-1 antigen (Mac-1) or cluster of differentiation molecule 11B (CD11B). It is mapped to 16p11.2. ITGAM has a role in vascular repair after mechanical arterial injury. It is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. What's more, ITGAM probably recognizes the R-G-D peptide in C3b, and it is also a receptor for fibrinogen, factor X and ICAM1.

Gene Full Name: Integrin alpha-M

Molecular Weight: 128 kDa

Gene ID: 16409

UniProt: P05555

Pathways: Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation