GAL4 antibody (C-Term)

Catalog No. ABIN4886660

The Rabbit Polyclonal anti-GAL4 antibody has been validated for WB, ICC, FACS and IF. It is suitable to detect GAL4 in samples from Human.

Quick Overview for GAL4 antibody (C-Term) (ABIN4886660)

Target: GAL4 (LGALS4) (Galectin 4 (LGALS4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GAL4 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-GAL4 Antibody

Binding Specificity: AA 283-320, C-Term

Purpose: Anti-GAL4/LGALS4 Antibody Picoband®

Sequence: DRFKVYANGQ HLFDFAHRLS AFQRVDTLEI QGDVTLSY

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-GAL4/LGALS4 Antibody Picoband® (ABIN4886660). Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human GAL4, different from the related mouse and rat sequences by seven amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. "Entrez Gene: LGALS4 lectin, galactoside-binding, soluble, 4 (galectin 4)". 2. Barondes SH, Cooper DN, Gitt MA, Leffler H (Sep 1994). "Galectins. Structure and function of a large family of animal lectins". J Biol Chem 269 (33): 20807-10. 3. Rechreche, H., Mallo, G. V., Montalto, G., Dagorn, J.-C., Iovanna, J. L. Cloning and expression of the mRNA of human galectin-4, an S-type lectin down-regulated in colorectal cancer. Europ. J. Biochem. 248: 225-230, 1997.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for GAL4

Target: GAL4 (LGALS4) (Galectin 4 (LGALS4))

Alternative Name: LGALS4

Background:

Synonyms: Galectin-4,Gal-4,Antigen NY-CO-27,L-36 lactose-binding protein,L36LBP,Lactose-binding lectin 4,LGALS4,

Background: Galectin-4 is a protein that in humans is encoded by the LGALS4 gene. This gene is mapped to chromosome 19q13.2 based on an alignment of the LGALS4 sequence. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. LGALS4 is an S-type lectin that is strongly underexpressed in colorectal cancer. The 323-amino acid LGALS4 protein contains 2 homologous, approximately 150-amino acid carbohydrate recognition domains and all amino acids typically conserved in galectins.

Molecular Weight: 39 kDa

Gene ID: 3960

UniProt: P56470