MAP3K7 antibody (C-Term)

Catalog No. ABIN4886662

This Rabbit Polyclonal antibody specifically detects MAP3K7 in WB, IF, ICC and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for MAP3K7 antibody (C-Term) (ABIN4886662)

Target: MAP3K7 (Mitogen-Activated Protein Kinase Kinase Kinase 7 (MAP3K7))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP3K7 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-MAP3K7 Antibody

Binding Specificity: AA 566-603, C-Term

Purpose: Anti-TAK1/MAP3K7 Antibody Picoband®

Sequence: RLVQEHKKLL DENKSLSTYY QQCKKQLEVI RSQQQKRQ

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-TAK1/MAP3K7 Antibody Picoband® (ABIN4886662). Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human TAK1, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. "Entrez Gene: MAP3K7 mitogen-activated protein kinase kinase kinase 7". 2. Kondo M, Osada H, Uchida K, Yanagisawa K, Masuda A, Takagi K, Takahashi T, Takahashi T (February 1998). "Molecular cloning of human TAK1 and its mutational analysis in human lung cancer". Int J Cancer 75 (4): 559-63. 3. Ninomiya-Tsuji J, Kishimoto K, Hiyama A, Inoue J, Cao Z, Matsumoto K (March 1999). "The kinase TAK1 can activate the NIK-I kappaB as well as the MAP kinase cascade in the IL-1 signalling pathway".Nature 398 (6724): 252-6.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for MAP3K7

Target: MAP3K7 (Mitogen-Activated Protein Kinase Kinase Kinase 7 (MAP3K7))

Alternative Name: MAP3K7

Background:

Synonyms: Mitogen-activated protein kinase kinase kinase 7,2.7.11.25,Transforming growth factor-beta-activated kinase 1,TGF-beta-activated kinase 1,MAP3K7,TAK1,

Tissue Specificity: Isoform 1A is the most abundant in ovary, skeletal muscle, spleen and blood mononuclear cells. Isoform 1B is highly expressed in brain, kidney and small intestine. Isoform 1C is the major form in prostate. Isoform 1D is the less abundant form. .

Background: Mitogen-activated protein kinase kinase kinase 7, also known as TAK1, is an enzyme that in humans is encoded by the MAP3K7 gene. The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase mediates the signaling transduction induced by TGF beta and morphogenetic protein (BMP), and controls a variety of cell functions including transcription regulation and apoptosis. In response to IL-1, this protein forms a kinase complex including TRAF6, MAP3K7P1/TAB1 and MAP3K7P2/TAB2, this complex is required for the activation of nuclear factor kappa B. This kinase can also activate MAPK8/JNK, MAP2K4/MKK4, and thus plays a role in the cell response to environmental stresses. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.

Sequence Similarities: Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC4 sub-subfamily.

Molecular Weight: 72 kDa

Gene ID: 6885

UniProt: O43318

Pathways: NF-kappaB Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Tube Formation, Toll-Like Receptors Cascades, BCR Signaling, Ubiquitin Proteasome Pathway