PF4 antibody (AA 30-105)

Catalog No. ABIN4886693

The Rabbit Polyclonal anti-PF4 antibody has been validated for WB, IHC and ELISA. It is suitable to detect PF4 in samples from Mouse.

Quick Overview for PF4 antibody (AA 30-105) (ABIN4886693)

Target: PF4 (Platelet Factor 4 (PF4))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PF4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-PF4 Antibody

Binding Specificity: AA 30-105

Purpose: Anti-PF4 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-PF4 Antibody Picoband® (ABIN4886693). Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived mouse PF4 recombinant protein (Position: V30-S105). Mouse PF4 shares 74.3% and 86.8% amino acid (aa) sequence identity with human and rat PF4, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Mouse
ELISA, 0.1-0.5 μg/mL, -
1. Eisman, R., Surrey, S., Ramachandran, B., Schwartz, E., Poncz, M. Structural and functional comparison of the genes for human platelet factor 4 and PF4-alt. Blood 76: 336-344, 1990. 2. Griffin, C. A., Emanuel, B. S., LaRocco, P., Schwartz, E., Poncz, M. Human platelet factor 4 gene is mapped to 4q12-q21. Cytogenet. Cell Genet. 45: 67-69, 1987. 3. Lambert, M. P., Rauova, L., Bailey, M., Sola-Visner, M. C., Kowalska, M. A., Poncz, M. Platelet factor 4 is a negative autocrine in vivo regulator of megakaryopoiesis: clinical and therapeutic implications. Blood 110: 1153-1160, 2007.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for PF4

Target: PF4 (Platelet Factor 4 (PF4))

Alternative Name: Pf4

Background:

Synonyms: Platelet factor 4,PF-4,C-X-C motif chemokine 4,Pf4,Cxcl4, Scyb4,

Tissue Specificity: Highly expressed in heart, placenta, pancreas, lung and liver and weakly expressed in spleen, lymph nodes and thymus. .

Background: Platelet factor 4 (PF4) is a small cytokine belonging to the CXC chemokine family that is also known as chemokine (C-X-C motif) ligand 4 (CXCL4). By in situ hybridization, the CXCL4 gene is mapped to chromosome 4q12-q21. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair.

Sequence Similarities: Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC4 sub-subfamily.

Molecular Weight: 11 kDa

Gene ID: 56744

UniProt: Q9Z126