PI3 antibody (AA 61-117)

Catalog No. ABIN4886694

The Rabbit Polyclonal anti-PI3 antibody (ABIN4886694) specifically detects PI3 in WB, IHC, ELISA, ICC, IF and FACS. The antibody is reactive with Human samples.

Quick Overview for PI3 antibody (AA 61-117) (ABIN4886694)

Target: PI3 (Peptidase Inhibitor 3, Skin-Derived (PI3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PI3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-PI3 Antibody

Binding Specificity: AA 61-117

Purpose: Anti-Elafin/Skalp/PI3 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Elafin/Skalp/PI3 Antibody (ABIN4886694). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human Elafin/Skalp recombinant protein (Position: A61-Q117).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for PI3

Target: PI3 (Peptidase Inhibitor 3, Skin-Derived (PI3))

Alternative Name: PI3

Background:

Synonyms: Elafin,Elastase-specific inhibitor,ESI,Peptidase inhibitor 3,PI-3,Protease inhibitor WAP3,Skin-derived antileukoproteinase,SKALP,WAP four-disulfide core domain protein 14,PI3,WAP3, WFDC14,

Tissue Specificity: Expressed by the liver and secreted in plasma.

Background: Elafin, also known as peptidase inhibitor 3 or skin-derived antileukoprotease (SKALP), is a protein that in humans is encoded by the PI3 gene. This gene encodes an elastase-specific inhibitor that functions as an antimicrobial peptide against Gram-positive and Gram-negative bacteria, and fungal pathogens. The protein contains a WAP-type four-disulfide core (WFDC) domain, and is thus a member of the WFDC domain family. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. And this gene belongs to the centromeric cluster. Expression of this gene is upgulated by bacterial lipopolysaccharides and cytokines.

Molecular Weight: 25 kDa

Gene ID: 5266

UniProt: P19957