ADRP antibody (AA 226-418)

Catalog No. ABIN4886697

This anti-ADRP antibody is a Rabbit Polyclonal antibody detecting ADRP in WB and IHC. Suitable for Human, Mouse and Rat.

Quick Overview for ADRP antibody (AA 226-418) (ABIN4886697)

Target: ADRP (PLIN2) (Perilipin 2 (PLIN2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADRP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-ADRP Antibody

Binding Specificity: AA 226-418

Purpose: Anti-ADFP/PLIN2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-ADFP/PLIN2 Antibody Picoband® (ABIN4886697). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human ADFP recombinant protein (Position: K226-Q418). Human ADFP shares 88.4% amino acid (aa) sequence identity with mouse ADFP.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Western blot, 0.1-0.5 μg/mL, Human
1. Heid, H. W., Schnolzer, M., Keenan, T. W. Adipocyte differentiation-related protein is secreted into milk as a constituent of milk lipid globule membrane. Biochem. J. 320: 1025-1030, 1996. 2. Shaw, C. S., Sherlock, M., Stewart, P. M., Wagenmakers, A. J. M.Adipophilin distribution and colocalisation with lipid droplets in skeletal muscle. Histochem. Cell Biol. 131: 575-581, 2009.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for ADRP

Target: ADRP (PLIN2) (Perilipin 2 (PLIN2))

Alternative Name: PLIN2

Background:

Synonyms: Perilipin-2,Adipophilin,Adipose differentiation-related protein,ADRP,PLIN2,ADFP,

Tissue Specificity: Milk lipid globules.

Background: Adipose differentiation-related protein, also known as perilipin 2 (PLIN2), ADRP or adipophilin, is a protein which in humans is encoded by the ADFP gene. The protein encoded by this gene belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene.

Molecular Weight: 48 kDa

Gene ID: 123

UniProt: Q99541

Pathways: Regulation of Lipid Metabolism by PPARalpha, Lipid Metabolism