D1 protein of PSII, DE- (PsbA) (AA 234-242) antibody

Catalog No. ABIN488673

Cited in 2+ publications. The Rabbit Polyclonal anti-D1 protein of PSII, DE- (PsbA) antibody (ABIN488673) specifically detects D1 protein of PSII, DE- (PsbA) in BN PAGE and WB. The antibody is reactive with Alfalfa, Arabidopsis thaliana, Barley, Chlamydomonas reinhardtii, Nannochloropsis oceanica, Neochloris oleoabundans, Nicotiana tabacum, Pea, Physcomytrium Patens, Silene vulgaris, Sinapis alba, Spinach, Synechococcus sp., Synechocystis and Wheat samples.

Quick Overview for D1 protein of PSII, DE- (PsbA) (AA 234-242) antibody (ABIN488673)

Target: D1 protein of PSII, DE- (PsbA)

Reactivity: Alfalfa, Arabidopsis thaliana, Barley, Chlamydomonas reinhardtii, Nannochloropsis oceanica, Neochloris oleoabundans, Nicotiana tabacum, Pea, Physcomytrium Patens, Silene vulgaris, Sinapis alba, Spinach, Synechococcus sp., Synechocystis, Wheat

Host: Rabbit

Clonality: Polyclonal

Application: Blue-native PAGE (BN PAGE), Western Blotting (WB)

Product Details

Binding Specificity: AA 234-242

Purpose: Anti-PsbA | D1 protein of PSII, DE-loop

Cross-Reactivity (Details): Arabidopsis thaliana, Chlamydomonas reinhardii, Hordeum vulgare, Medicago truncatula, Nannochloropsis oceanica strain IMET1, Neochloris oleoabundans UTEX 1185 (chlorophyta), Nicotiana tabacum, Physcomitrium patens, Pisum sativum, Silene vulgaris, Sinapsis alba, Spinacia oleracea, Synechococcus sp. PCC 7942, Synechocystis sp. PCC6803, Triticum sp.
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Predicted Reactivity: Cucumis sativus,Glycine max,Nannochloropsis sp.,Oryza sativa,Populus balsamifera,Ricinus communis,Zea mays,Vitis vinifera

Purification: Serum

Immunogen: KLH-conjugated synthetic peptide

Application Details

Application Notes: 1 : 10 000, thylakoid fraction (WB)

Comment:

antibody is recognizing a 23 kDa fragment in spinach and Arabidopsis thylakoidsfor usage on total cell extracts the dilution needs to be determined experimentally

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Serum

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

References

Sobrino-Plata, Carrasco-Gil, Abadía, Escobar, Álvarez-Fernández, Hernández: "The role of glutathione in mercury tolerance resembles its function under cadmium stress in Arabidopsis." in: Metallomics : integrated biometal science, Vol. 6, Issue 2, pp. 356-66, (2014) (PubMed).

Yoshioka, Nakayama, Yoshida, Ohashi, Morita, Kobayashi, Yamamoto: "Quality control of photosystem II: FtsH hexamers are localized near photosystem II at grana for the swift repair of damage." in: The Journal of biological chemistry, Vol. 285, Issue 53, pp. 41972-81, (2010) (PubMed).

Target Details

Target: D1 protein of PSII, DE- (PsbA)

Alternative Name: D1 Protein of PSII, DE (PsbA)

Background:

Background: The psbA gene has been cloned from many species of plants, green algae, and cyanobacteria. The psbA gene is located in the chloroplast genome and encodes for the D1 protein, a core component of Photosystem II. PsbA/D1 is rapidly cycled under illumination in all oxygenic photobionts. Tracking PsbA pools using the Global PsbA antibody can show the functional content of Photosystem II in a wide range of samples. Alternative names: 32 kDa thylakoid membrane protein, photosystem II protein D1

Additional information: Antibody will detect 23 kDa N-terminal fragment

Molecular Weight: 38 | 28-30 kDa

Gene ID: 844802

UniProt: P83755