VASP antibody (N-Term)

Catalog No. ABIN4886759

The Rabbit Polyclonal anti-VASP antibody is suitable to detect VASP in samples from Human and Rat. It has been validated for WB and IHC.

Quick Overview for VASP antibody (N-Term) (ABIN4886759)

Target: VASP (Vasodilator-Stimulated phosphoprotein (VASP))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VASP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-VASP Antibody

Binding Specificity: AA 78-114, N-Term

Purpose: Anti-VASP Antibody

Sequence: NFHQWRDARQ VWGLNFGSKE DAAQFAAGMA SALEALE

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-VASP Antibody (ABIN4886759). Tested in IHC, WB applications. This antibody reacts with Human, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human VASP, different from the related mouse sequence by four amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Rat

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for VASP

Target: VASP (Vasodilator-Stimulated phosphoprotein (VASP))

Alternative Name: VASP

Background:

Synonyms: Vasodilator-stimulated phosphoprotein,VASP,VASP,

Tissue Specificity: Highly expressed in platelets. .

Background: Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG.

Molecular Weight: 40 kDa

Gene ID: 7408

UniProt: P50552

Pathways: TCR Signaling, Regulation of Actin Filament Polymerization, Tube Formation