YAP1 antibody (N-Term)

Catalog No. ABIN4886772

This anti-YAP1 antibody is a Rabbit Polyclonal antibody detecting YAP1 in WB, IHC, IF, FACS, IP and ICC. Suitable for Human, Mouse and Rat.

Quick Overview for YAP1 antibody (N-Term) (ABIN4886772)

Target: YAP1 (Yes-Associated Protein 1 (YAP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This YAP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-YAP1 Antibody

Binding Specificity: AA 62-97, N-Term

Purpose: Anti-YAP1 Antibody Picoband®

Sequence: ETDLEALFNA VMNPKTANVP QTVPMRLRKL PDSFFK

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-YAP1 Antibody Picoband® (ABIN4886772). Tested in Flow Cytometry, IP, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human YAP1, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Immunoprecipitation, 0.5-2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Huang, J., Wu, S., Barrera, J., Matthews, K., Pan, D. (2005). "The Hippo signaling pathway coordinately regulates cell proliferation and apoptosis by inactivating Yorkie, the Drosophila Homolog of YAP". Cell122 (3): 421-34. 2. Overholtzer M, Zhang J, Smolen GA, Muir B, Li W, Sgroi DC, Deng CX, Brugge JS, Haber DA. (2006). "Transforming properties of YAP, a candidate oncogene on the chromosome 11q22 amplicon". Proc Natl Acad Sci U S A. 103 (33): 12405-12410. 3. Sudol M (1994). "Yes-associated protein (YAP65) is a proline-rich phosphoprotein that binds to the SH3 domain of the Yes proto-oncogene product". Oncogene 9 (8): 2145-2152.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for YAP1

Target: YAP1 (Yes-Associated Protein 1 (YAP1))

Alternative Name: YAP1

Background:

Synonyms: Transcriptional coactivator YAP1,Yes-associated protein 1,Protein yorkie homolog,Yes-associated protein YAP65 homolog,YAP1,YAP65,

Tissue Specificity: Increased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level). .

Background: YAP1, also known as YAP or YAP65, is a potent oncogene, which is amplified in various human cancers. This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. It is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms.

Molecular Weight: 75 kDa

Gene ID: 10413

UniProt: P46937

Pathways: MAPK Signaling, Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha