ZP1 antibody (N-Term)

Catalog No. ABIN4886775

Product Details anti-ZP1 Antibody

Target: ZP1 (Zona Pellucida Glycoprotein 1 (ZP1))

Binding Specificity: AA 102-139, N-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZP1 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Anti-ZP1 Antibody Picoband®

Sequence: HVLEKDGRFH LRVFMEAVLP NGRVDVAQDA TLICPKPD

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-ZP1 Antibody Picoband® (ABIN4886775). Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human ZP1, different from the related mouse sequence by four amino acids, and from the related rat sequence by five amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Rat
1. Gross, M. B. Personal Communication. Baltimore, Md. 6/24/2014. 2. Huang, Hua-Lin, Lv, Chao, Zhao, Ying-Chun, Li, Wen, He, Xue-Mei, Li, Ping, Sha, Ai-Guo, Tian, Xiao, Papasian, Christopher J., Deng, Hong-Wen, Lu, Guang-Xiu, Xiao, Hong-Mei (2014). "Mutant ZP1 in Familial Infertility". New England Journal of Medicine 370 (13): 1220-1226. 3. Rankin, T., Talbot, P., Lee, E., Dean, J. Abnormal zonae pellucidae in mice lacking ZP1 result in early embryonic loss.Development 126: 3847-3855, 1999.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for ZP1

Target: ZP1 (Zona Pellucida Glycoprotein 1 (ZP1))

Alternative Name: ZP1 (ZP1 Products)

Background:

Synonyms: Zona pellucida sperm-binding protein 1,Zona pellucida glycoprotein 1,Zp-1,Processed zona pellucida sperm-binding protein 1,ZP1,

Tissue Specificity: Oocytes.

Background: Zona pellucida sperm-binding protein 1 (ZP1) is a protein that belopngs to the mammalian zona pellucida. The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. Zp1 ensures the structural integrity of the zona pellucida. Mutations in this gene are a cause of oocyte maturation defect and infertility. And this gene is mapped to chromosome 11q12.2.

Molecular Weight: 70 kDa, 100 kDa

Gene ID: 22917

UniProt: P60852